| Sexually dimorphic, androgen sensitive, enkephalinergic afferents to a lumbar motor nucleus of rats. | |
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MedLine Citation:
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PMID: 1779226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In male rats, methionine-enkephalin immunoreactivity (enkephalin-ir) has been observed in the dorsal lateral nucleus (DLN), a longitudinal pool of motoneurons in the lumbar spinal cord. Within the DLN a mediodorsal crescent of intense enkephalin-ir staining surrounds the motoneurons innervating the ischiocavernosus muscle of the penis, which suggests a function of the enkephalinergic afferents in male copulatory activities. The present study attempted to determine the roles of gender and adult exposure to androgen in shaping the striking subnuclear distribution of enkephalin-ir. Transverse sections through L5-6 were obtained from mature male and female rats that were gonadally intact, gonadectomized, or gonadectomized and treated with testosterone, as well as from male rats genetically deficient in androgen receptors (Tfm). The sections were incubated with primary antiserum raised against methionine enkephalin and bound antibodies were visualized using the avidin-biotin-peroxidase technique. A microphotometer was used to compare the staining density in laminae I-II of the dorsal horn, ventral grey matter, and the DLN. In all groups the DLN stained more darkly than the ventral grey, demonstrating the presence of enkephalin-ir in the DLN regardless of gender or exposure to androgen. However, the mediodorsal crescent of dense staining in the DLN was obvious only in gonadally intact males, while the entire DLN stained darkly in both sexes of gonadectomized rats treated with androgen. Therefore, the preferential distribution of enkephalin-ir in the mediodorsal crescent of the DLN is sexually dimorphic though the overall content of enkephalin-ir within the DLN responds to androgen. |
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Authors:
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A Coquelin; P E Micevych; A P Arnold |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurobiology Volume: 22 ISSN: 0022-3034 ISO Abbreviation: J. Neurobiol. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-03-12 Completed Date: 1992-03-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0213640 Medline TA: J Neurobiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 873-81 Citation Subset: IM |
Affiliation:
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Department of Anatomy, University of California, Los Angeles 90024. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Castration Copulation / physiology* Enkephalin, Methionine / analysis*, physiology Female Immunoenzyme Techniques Male Penis / innervation* Rats / anatomy & histology*, physiology Rats, Inbred Strains / anatomy & histology Rats, Mutant Strains Receptors, Androgen / drug effects Sex Characteristics* Spinal Cord / anatomy & histology*, chemistry, physiology Testosterone / pharmacology, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HD15021/HD/NICHD NIH HHS; HD18859/HD/NICHD NIH HHS; NS21220/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Androgen; 58-22-0/Testosterone; 58569-55-4/Enkephalin, Methionine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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