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Sexual fusion of protoplasts in a marine green alga, Bryopsis plumosa.
MedLine Citation:
PMID:  22228469     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We isolated protoplasts from male and female gametophytes of a strictly dioecious strain of the coenocytic marine green alga Bryopsis plumosa. The protoplasts successfully developed into macrothalli. These in turn produced swimming cells, which appeared similar to biflagellated gametes even when the mixed protoplasts were comprised of protoplasm from male and female gametophytes. We found that swimming cell sizes depended on the male/female protoplasm ratio; macrothalli successfully produced swimming cells with male/female protoplasm ratios of 10:0; 9:1; 7:3; 5:5; 1:9; and 0:10. In male/female protoplasm ratios ranging from equal to strongly female biased (5:5; 3:7; 1:9), swimming cells exhibited normal behaviors of gametes and resultant zygotes, displaying positive and negative phototaxis, respectively. Negatively phototactic swimming cells were quadriflagellated and had two nuclei, apparently as a result of fusion, but never developed into microthalli. Thus, these swimming cells might lack functionality essential for normal gametes. Our findings suggested that natural monoecy observed in this genus did not originate from hybridization of protoplasm between the sexes.
Authors:
Tatsuya Togashi; Kaori Sakakibara; Makoto Nozawa; Paul Alan Cox
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-7
Journal Detail:
Title:  Sexual plant reproduction     Volume:  -     ISSN:  1432-2145     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955730     Medline TA:  Sex Plant Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Marine Biosystems Research Center, Chiba University, Kamogawa, 299-5502, Japan, togashi@faculty.chiba-u.jp.
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