| Sexual dimorphism in LEC rat liver: suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis. | |
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MedLine Citation:
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PMID: 21551946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined age-related changes in the protein expression of carbonic anhydrase III (CAIII) in livers of Long-Evans with a cinnamon-like color (LEC) rats using an agouti color (LEA) rats as controls. The levels of the protein of CAIII in the liver of LEC male rats increased before 20 weeks of age, at the stage of acute hepatitis, and were decreased at 54 weeks of age, while those of CAIII in the liver of LEA male rats were highly expressed at all ages. In the normal LEA rats, CAIII showed sexual dimorphism. The level of CAIII in LEA male rat liver relative to female was four times higher. On the other hand, young LEC rat (at 4-12 weeks) showed a higher protein level of CAIII than LEA rats, and then decreased during development of hepatitis. CAIII mRNA also decreased in the LEC rat liver during hepatocarcinogenesis. The level of CAIII in the tumor region was lower than that in the tumor-free region. Immunohistochemical analysis showed that glutathione S-transferase P (GST-P) was positive and CAIII was negative in the precancerous region. The expression of CAIII was suppressed in cancerous lesions in hepatoma-bearing LEC rat liver compared to uninvolved surrounding tissues. These results indicated that suppression of CAIII accompanied hepatocarcinogenesis and it is a secondary consequence of the high copper levels in the liver. |
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Authors:
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Makihiko Kuhara; Jingshu Wang; Maria Jolina Flores; Zhiwei Qiao; Yukio Koizumi; Souichi Koyota; Naoyuki Taniguchi; Toshihiro Sugiyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical research (Tokyo, Japan) Volume: 32 ISSN: 1880-313X ISO Abbreviation: Biomed. Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-05-09 Completed Date: 2011-09-09 Revised Date: 2011-12-05 |
Medline Journal Info:
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Nlm Unique ID: 8100317 Medline TA: Biomed Res Country: Japan |
Other Details:
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Languages: eng Pagination: 111-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Blotting, Western Carbonic Anhydrase III / analysis, antagonists & inhibitors, biosynthesis* Carcinoma, Hepatocellular* Cell Transformation, Neoplastic / genetics, metabolism Copper* / adverse effects, metabolism Female Glutathione Transferase / analysis, biosynthesis Hepatitis / etiology, genetics, metabolism, pathology Immunohistochemistry Liver / metabolism, pathology* Liver Neoplasms / etiology, genetics, metabolism*, pathology Male RNA, Messenger / analysis Rats Rats, Inbred LEC / genetics*, metabolism Rats, Inbred Strains / genetics, metabolism Sex Factors |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 7440-50-8/Copper; EC 2.5.1.18/Glutathione Transferase; EC 4.2.1.-/Carbonic Anhydrase III |
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