| Sexual dimorphism among calbindin-D28K immunoreactive cells in the rat pineal body. | |
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MedLine Citation:
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PMID: 8163388 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Calbindin antibodies have been used in neuroanatomical studies to give excellent cytoarchitectural staining and visualization of a Golgi-like cellular morphology. Calbindin-D28K immunoreactivity used in rat pineal gland as a marker detected two classes of pineal cells. One class of small cells representing exclusively glial cells was strongly immunoreactive, and presented a large variety of individual shapes. The majority were a pyramidal shape with one or more processes while others displayed a cytoplasmic lipid droplet. Some small cells occurred around pericapillary spaces. The second class of calbindin-D28K positive cells corresponding to type II pinealocytes were characterized by their large size and less intensive labelling. Type II pinealocytes were round or rectangular; the nucleus was infolded and large with a prominent nucleolus. These large cells were preferentially distributed in the vicinity of vessels and assembled in a cluster of more than ten cells. The lack of S-100 and myeloperoxidase immunoreactivities in large calbindin-D28K cells excluded their possible characterization as glial cells and mononuclear phagocytes, while their size (> 15 microns) excluded microglial cells. A sex difference was detected between large calbindin-D28K positive cells. The mean calculated number of large positive cells for males was 6361 +/- 1504 (n = 8) compared to 2162 +/- 1235 (n = 7) for females. No significative difference was detected between males and females for small calbindin-D28K positive cells. |
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Authors:
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E Bastianelli; R Pochet |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histochemistry Volume: 100 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-05-26 Completed Date: 1994-05-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY |
Other Details:
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Languages: eng Pagination: 449-55 Citation Subset: IM |
Affiliation:
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Laboratoire d'Histologie, Faculté de Médecine, Université libre de Bruxelles, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Calcium-Binding Protein, Vitamin D-Dependent / metabolism* Female Immunohistochemistry Male Peroxidase / metabolism Pineal Gland / cytology, metabolism* Rats Rats, Wistar S100 Proteins / metabolism Sex Characteristics |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Calcium-Binding Protein, Vitamin D-Dependent; 0/S100 Proteins; 0/calbindin; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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