Document Detail


Sexual differences in branched chain amino acid metabolism into fatty acids and cholesterol in Harderian gland of golden hamster.
MedLine Citation:
PMID:  9722679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Harderian gland of golden hamster (Mesocricetus auratus) secretes copious lipids, most of which is 1-alkyl-2,3-diacylglycerol (ADG). We previously reported that the composition of ADG shows marked sexual dimorphism [Seyama et al. (1995) J. Biochem. 117, 661-670]. Male ADG contains only straight chain alkyl and acyl groups, but female ADG contains a lot of branched chain ones too. In this study, we investigated the metabolism of branched chain amino acids (BCAAs) and analyzed the incorporation of the metabolites into lipids in the Harderian gland. Golden hamsters were injected intraperitoneally with [U-14C]BCAAs, and Harderian glands were obtained at 3, 6, 9, and 24 h after injection. Lipids were then extracted from the glands and analyzed. Thin layer chromatography revealed that the ADG was labeled in both sexes, but the profile depended on the sex. The cholesterol fraction was labeled only in the male gland. The alkyl and acyl groups of ADG were subjected to radio-gas liquid chromatography. As for the alkyl groups, radioactivity was detected in straight-C16 and -C18 chains in males, while branched-C17 and -C19 chains were labeled in females. As for the acyl groups, straight-C14, -C15, and -C16 chains were labeled in males, while in females, branched-C17 and -C19 chains were labeled as well as a straight-C16 chain. These results suggest that the BCAA metabolism should be regulated as to the sex at the step of branched chain acyl-CoA degradation in the Harderian gland of golden hamster, which causes the sexual dimorphism in the lipid composition in this gland.
Authors:
A Hida; Y Uchijima; Y Seyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  124     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-11     Completed Date:  1998-12-11     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  648-53     Citation Subset:  IM    
Affiliation:
Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry,  metabolism*
Animals
Cholesterol / metabolism*
Chromatography, Gas
Chromatography, Thin Layer
Cricetinae
Cricetulus
Fatty Acids / metabolism*
Female
Harderian Gland / metabolism*
Male
Mesocricetus
Radiometry
Sex Factors
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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