Document Detail


Sex steroids regulate epithelial-stromal cell cross-talk and trophoblast attachment-invasion in a three-dimensional human endometrial culture system.
MedLine Citation:
PMID:  23320930     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Human embryo implantation involves a complex network of molecular signaling that is modulated by endocrine and paracrine pathways. Here, we performed studies using a unique and recently developed three dimensional (3D) implantation model, characterized by an endometrium-like 3D culture system and Jar cells-derived spheroids mimicking the embryo/trophoblast. The aims were to investigate the effects of 17β estradiol (E2) and medroxyprogesterone acetate (MPA) on (i) the interaction between epithelial and stromal cells, and (ii) the attachment and invasion of trophoblast cells. We observed that epithelial and stromal cells in the 3D culture were ERα+, ERβ+, and PR+. Decidualization was confirmed by enhanced prolactin gene expression on day 7 of E2 plus MPA treatment. An effect of epithelial cells on the decidualization of stromal cells was indicated by significantly higher levels of prolactin mRNA expression in the 3D culture compared to stromal cells grown within the fibrin-agarose gel matrix. On the other hand, the relative gene expressions of E-cadherin and IL-1ß in epithelial cells of the 3D culture under decidualization conditions significantly differed from those in epithelial cells grown over the fibrin-agarose gel matrix without stromal cells, pointing to regulation of epithelial cells by the stroma. The attachment rate of Jar spheroids to the 3D was significantly increased by E2 plus MPA treatment. Analyses of Z-stack confocal and stained optic microscopic images demonstrated that Jar spheroids breached the epithelial cell monolayer, invaded, and were embedded into the 3D matrix in response to decidualization signals. In summary, the newly bioengineered system provides a unique model for studying interactions between the different endometrial cell compartments, via soluble-paracrine signals as well as cell to cell interactions, and is a useful tool to study early embryonic implantation events.
Authors:
Hai Wang; Silvina Bocca; Sandra Anderson; Liang Yu; Bhaskara S Rhavi; Jose Horcajadas; Sergio Oehninger
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-16
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  -     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
The Jones Institute for Reproductive Medicine, Obstetrics and Gynecology, Norfolk, Virginia, United States; haiwangz@yahoo.cn.
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