| Sex-specific differences in rabbit fetal lung maturation in response to epidermal growth factor. | |
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MedLine Citation:
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PMID: 1731954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Males and females exhibit different stages of lung development at the same gestation with males lagging behind. We hypothesized that one of the mechanisms responsible for the sex-specific difference in fetal lung maturation is a delay in the onset of epidermal growth factor (EGF) activity in the male fetal lung. EGF influences growth and differentiation during development. We studied the effects of EGF on the incorporation of glycerol into lamellar body disaturated phosphatidylcholine (DSPC) in sex-specific fetal rabbit lung explants prepared at 21 and 24 days gestation (term 31 days). The explants were maintained in Waymouth's media + 10% stripped fetal calf serum with or without EGF (10 ng/ml). The incorporation of [1,3-14C]glycerol into lamellar body DSPC was assessed after 3, 5, or 7 days of culture. Female lung explants prepared at 21 days of gestation had increased incorporation of glycerol into DSPC over time in response to EGF treatment. Male lung explants prepared at 21 days did not respond to EGF treatment. In explants prepared at 24 days gestation, baseline glycerol incorporation into DSPC was higher in female as compared to male fetal lung explants. EGF-responsiveness was also sex-specific in these more mature explants, with the male explants now responding to EGF with a consistent increase in the incorporation of glycerol into lamellar body DSPC. We conclude that one of the mechanisms responsible for the lag in male fetal lung development is a delay in the onset of EGF activity. |
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Authors:
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J M Klein; H C Nielsen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1133 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1992 Jan |
Date Detail:
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Created Date: 1992-02-21 Completed Date: 1992-02-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 121-6 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, New England Medical Center, Boston, MA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Epidermal Growth Factor / pharmacology* Female Fetal Organ Maturity / drug effects* Glycerol / metabolism Lung / drug effects, embryology*, metabolism Male Organ Culture Techniques Phosphatidylcholines / biosynthesis Pregnancy Rabbits Sex Characteristics* |
| Grant Support | |
ID/Acronym/Agency:
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HL37930/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 56-81-5/Glycerol; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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