Document Detail

Sex differences in cardio-respiratory transition and surfactant composition following preterm birth in sheep.
MedLine Citation:
PMID:  22914752     Owner:  NLM     Status:  Publisher    
Male preterm infants are at greater risk of respiratory morbidity and mortality than females but mechanisms are poorly understood. Our objective was to identify the basis for the "male disadvantage" following preterm birth using an ovine model of preterm birth in which survival of females is greater than males. At 0.85 of term, fetal sheep underwent surgery (11 female, 10 male) for the implantation of vascular catheters to monitor blood gases and arterial pressure. After caesarean delivery at 0.90 of term, lambs were monitored for 4h while spontaneously breathing; lambs were then euthanized and static lung compliance measured. We analysed surfactant phospholipid composition in amniotic fluid and in bronchoalveolar lavage fluid (BALF) taken at necropsy; we also analysed surfactant protein (SP) expression in lung tissue. Before delivery male fetuses tended to have lower pH (p=0.052) compared to females. One hour after delivery, males had significantly lower pH and higher PaCO(2), lactate, glucose and mean arterial pressure than females. Two males died 1 hour after birth. Static lung compliance was 37% lower in males than females (p<0.05). In BALF, males had significantly more protein, a lower percentage of the phosphatidylcholine (PC) 32:0 (dipalmitoylphosphatidylcholine) and higher percentages of PC34:2 and PC36:2. There were no sex-related differences in lung architecture or expression of SP-A, -B, -C and -D. The lower lung compliance in male preterm lambs compared to females may be due to altered surfactant phospholipid composition and function. These changes may compromise gas exchange and impair respiratory adaptation after male preterm birth.
Noreen Ishak; Takushi Hanita; Foula Sozo; Gert S Maritz; Richard Harding; Robert De Matteo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-22
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  -     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Monash University.
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