Document Detail


Sex determination and milk protein genotyping of preimplantation stage bovine embryos using multiplex PCR.
MedLine Citation:
PMID:  16727195     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A method for determining the sex and milk protein genotypes (RFLPs) of preimplantation stage bovine embryos using multiplex polymerase chain reaction (PCR) is described. Day 6 to 7 embryos were micromanipulated to isolate 5 to 6 cells. These cells were then dried in reaction tubes for transport to the laboratory. Subsequently, two sets of PCRs were performed using Y chromosome, k-casein and beta-lactoglobulin gene specific primers, followed by electrophoretic analysis of the PCR products. The presence or absence of the Y chromosome was ascertained in 90 of 92 embryos. Moreover, the k-casein specific fragment was amplified and detected in all these embryos. The PCR products were digested in order to genotype the k-casein gene. In 70% of the embryos, the beta-lactoglobulin specific fragment was amplified, although together with some unspecific fragments.
Authors:
P L Agrawala; V A Wagner; H Geldermann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  38     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1992 Nov 
Date Detail:
Created Date:  2006-05-26     Completed Date:  2009-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  969-78     Citation Subset:  -    
Affiliation:
Institute of Animal Husbandry and Breeding, Deptartment of Animal Breeding (470), University of Hohenheim, 7000 Stuttgart 70, Germany.
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