Document Detail


Sex peptide is required for the efficient release of stored sperm in mated Drosophila females.
MedLine Citation:
PMID:  20679516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Drosophila seminal fluid protein (SFP) sex peptide (SP) elicits numerous post-mating responses, including increased egg laying and decreased sexual receptivity, in the mated female. Unlike other SFPs, which are detectable in mated females for only a few hours post mating, SP is maintained-and its effects are sustained-for several days. The persistence of SP in the mated female's reproductive tract is thought to be a consequence of its binding to, and gradual release from, sperm in storage, which maintains SP's ability to act within the female reproductive tract. Recent studies have shown that several other SFPs, acting in a network, are needed for SP's localization to sperm and are necessary for the efficient release of sperm from storage. This result suggested an additional new role for SP modulating the release of sperm from storage. We tested for this possibility by examining sperm storage parameters in mated females that did not receive SP. We found that while sperm accumulation into storage was unaffected, sperm depletion from storage sites was significantly decreased (or impaired) in the absence of SP. Mates of males expressing a modified SP that is unable to be released from sperm showed a similar phenotype, indicating that release of sperm-bound SP is a necessary component of normal sperm depletion. Additionally, SP null males were more successful in a sperm competitive environment when they were first to mate, which is likely a consequence of higher retention of their sperm due to defective sperm release. Our findings illustrate a direct role for SP in the release of sperm from storage.
Authors:
Frank W Avila; K Ravi Ram; Margaret C Bloch Qazi; Mariana F Wolfner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-08-02
Journal Detail:
Title:  Genetics     Volume:  186     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-13     Completed Date:  2011-03-21     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  595-600     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila Proteins / genetics,  physiology*
Drosophila melanogaster / genetics,  physiology*
Female
Male
Mutation
Oviposition
Peptides / genetics,  physiology*
Reproduction
Semen / physiology*
Spermatozoa / physiology*
Grant Support
ID/Acronym/Agency:
R01 HD038921/HD/NICHD NIH HHS; R01-HD038921/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Peptides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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