Document Detail


Sex of muscle stem cells does not influence potency for cardiac cell therapy.
MedLine Citation:
PMID:  19523348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that populations of skeletal muscle-derived stem cells (MDSCs) exhibit sex-based differences for skeletal muscle and bone repair, with female cells demonstrating superior engrafting abilities to males in skeletal muscle while male cells differentiating more robustly toward the osteogenic and chondrogenic lineages. In this study, we tested the hypothesis that the therapeutic capacity of MDSCs transplanted into myocardium is influenced by sex of donor MDSCs or recipient. Male and female MDSCs isolated from the skeletal muscle of 3-week-old mice were transplanted into recipient male or female dystrophin-deficient (mdx) hearts or into the hearts of male SCID mice following acute myocardial infarction. In the mdx model, no difference was seen in engraftment or blood vessel formation based on donor cell or recipient sex. In the infarction model, MDSC-transplanted hearts showed higher postinfarction angiogenesis, less myocardial scar formation, and improved cardiac function compared to vehicle controls. However, sex of donor MDSCs had no significant effects on engraftment, angiogenesis, and cardiac function. VEGF expression, a potent angiogenic factor, was similar between male and female MDSCs. Our results suggest that donor MDSC or recipient sex has no significant effect on the efficiency of MDSC-triggered myocardial engraftment or regeneration following cardiac injury. The ability of the MDSCs to improve cardiac regeneration and repair through promotion of angiogenesis without differentiation into the cardiac lineage may have contributed to the lack of sex difference observed in these models.
Authors:
Lauren Drowley; Masaho Okada; Thomas R Payne; Gregory P Botta; Hideki Oshima; Bradley B Keller; Kimimasa Tobita; Johnny Huard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-05-06
Journal Detail:
Title:  Cell transplantation     Volume:  18     ISSN:  1555-3892     ISO Abbreviation:  Cell Transplant     Publication Date:  2009  
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1137-46     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Lineage
Dystrophin / deficiency,  genetics,  metabolism
Female
Heart / physiology
Male
Mice
Mice, Inbred C57BL
Mice, SCID
Muscle, Skeletal / cytology*
Myocardial Infarction / therapy*
Myocardium / pathology
Sex Factors
Stem Cell Transplantation*
Vascular Endothelial Growth Factor A / metabolism
Grant Support
ID/Acronym/Agency:
HL 069368/HL/NHLBI NIH HHS; IU54AR050733-01/AR/NIAMS NIH HHS; T32 EB001026-05/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Dystrophin; 0/Vascular Endothelial Growth Factor A

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