| Sevoflurane postconditioning protects chronically-infarcted rat hearts against ischemia-reperfusion injury by activation of pro-survival kinases and inhibition of mitochondrial permeability transition pore opening upon reperfusion. | |
| | |
MedLine Citation:
|
PMID: 19881297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated the cardioprotection against myocardial ischemia-reperfusion injury induced by sevoflurane postconditioning (SpostC) in chronically-infarcted rat hearts, and investigated the roles of phosphoinositide 3-kinase (PI3K)-protein kinase B/Akt (PKB/Akt), mitogen-activated extracellular regulated kinase 1/2 (MEK 1/2)-extracellular regulated kinase 1/2 (ERK 1/2), and mitochondrial permeability transition pore (mPTP). Left anterior descending (LAD) coronary artery was ligated to induce myocardial infarction in rats. Six weeks later, chronically-infarcted hearts were isolated and subjected to 30 min of global ischemia, followed by 1 h of reperfusion with Krebs-Henseleit (K-H) buffer. SpostC was administered by perfusing the hearts with K-H buffer saturated with 3% sevoflurane during the first 15 min of reperfusion. To evaluate the role of PI3K-PKB/Akt and MEK 1/2-ERK 1/2 in SpostC, PI3K inhibitor LY294002 (15 microM) and MEK 1/2 inhibitor PD98059 (20 microM) were administered alone or together with sevoflurane during the first 15 min of reperfusion. We found that exposure of 3% sevoflurane during early reperfusion significantly improved functional recovery (improved left ventricular developed pressure (LVDP), +/-dp/dt, CF, HR and reduced left ventricular end-diastolic pressure (LVEDP)), decreased myocardial infarct size and reduced LDH and CK-MB release, when compared with unprotected hearts. However, these protective effects were abolished in the presence of either LY294002 or PD98059, which was accompanied by the prevention of PKB/Akt and ERK 1/2 phosphorylation, and reduction of myocardial nicotinamide adenine dinucleotide (NAD+) content. These findings suggest that sevoflurane postconditioning protects chronically-infarcted rat hearts against ischemia-reperfusion injury by inhibiting mPTP opening via recruitment of PKB/Akt and ERK 1/2. |
| | |
Authors:
|
Yuntai Yao; Lihuan Li; Libing Li; Changqing Gao; Chunxia Shi |
Related Documents
:
|
2159727 - Txa2 inhibition and ischemia-induced loss of myocardial function and reactive hyperemia. 17283277 - Critical role of monocyte chemoattractant protein-1/cc chemokine ligand 2 in the pathog... 11665797 - Caspase-dependent and serine protease-dependent dna fragmentation of myocytes in the is... 1937987 - Coronary venous noradrenaline during coronary angioplasty. 7876047 - Effect of intravenous streptokinase on left ventricular function following acute myocar... 17948087 - A synopsis of research in cardiac apoptosis and its application to congestive heart fai... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biological & pharmaceutical bulletin Volume: 32 ISSN: 1347-5215 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-11-02 Completed Date: 2010-02-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
|
Languages: eng Pagination: 1854-61 Citation Subset: IM |
Affiliation:
|
Department of Anesthesiology, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, No. 167 Beilishi Road, Xicheng District, Beijing 100037, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anesthetics, Inhalation
/
pharmacology* Animals Blotting, Western Enzyme Activation Male Methyl Ethers / pharmacology* Mitochondria, Heart / drug effects* Myocardial Infarction / complications* Permeability Protein Kinases / metabolism* Rats Rats, Sprague-Dawley Reperfusion Injury / prevention & control* |
| Chemical | |
Reg. No./Substance:
|
0/Anesthetics, Inhalation; 0/Methyl Ethers; 28523-86-6/sevoflurane; EC 2.7.-/Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inductive potential of recombinant human granulocyte colony-stimulating factor to mature neutrophils...
Next Document: Effects of anti-dementia drugs on morphine-induced somnolence.