| Sevoflurane Protects Ventricular Myocytes from Ca2+ Paradox-Mediated Ca2+ Overload by Blocking the Activation of Transient Receptor Potential Canonical Channels. | |
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MedLine Citation:
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PMID: 21799395 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND:: Volatile anesthetics produce cardioprotective action by attenuating cellular Ca overload. The Ca paradox is an important model for studying the mechanisms associated with Ca overload-mediated myocardial injury, and was recently found to be mediated by Ca entry through the transient receptor potential canonical channels upon Ca repletion. This study investigated the effect of sevoflurane on cellular mechanisms underlying the Ca paradox. METHODS:: The Ca paradox was examined in fluo-3 or mag-fluo-4-loaded mouse ventricular myocytes using confocal laser scanning microscope, upon Ca repletion after 15 min of Ca depletion in the absence and presence of sevoflurane. RESULTS:: The Ca paradox was evoked in approximately 65% of myocytes upon Ca repletion, as determined by an abrupt elevation of cytosolic Ca accompanied by hypercontracture. The Ca paradox was significantly suppressed by sevoflurane administered for 3 min before and during Ca repletion (Post) or during Ca depletion and repletion (Postlong), and Postlong was more beneficial than Post application. The sarcoplasmic reticulum Ca levels gradually decreased during Ca depletion, and the Ca paradox was readily evoked in myocytes with reduced sarcoplasmic reticulum Ca levels. Postlong but not Post application of sevoflurane prevented decrease in sarcoplasmic reticulum Ca levels by blocking Ca leak through ryanodine receptors. Whole cell patch-clamp recordings revealed that sevoflurane rapidly blocked thapsigargin-induced transient receptor potential canonical currents. CONCLUSIONS:: Sevoflurane protects ventricular myocytes from Ca paradox-mediated Ca overload by blocking transient receptor potential canonical channels and by preventing the decrease in sarcoplasmic reticulum Ca levels, which is associated with less activation of transient receptor potential canonical channels. |
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Authors:
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Akiko Kojima; Hirotoshi Kitagawa; Mariko Omatsu-Kanbe; Hiroshi Matsuura; Shuichi Nosaka |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-27 |
Journal Detail:
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Title: Anesthesiology Volume: - ISSN: 1528-1175 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1300217 Medline TA: Anesthesiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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* Assistant Professor, Department of Anesthesiology, Shiga University of Medical Science, Otsu, Shiga, Japan. † Associate Professor, Department of Physiology, Shiga University of Medical Science. ‡ Professor, Department of Physiology, Shiga University of Medical Science. § Professor, Department of Anesthesiology, Shiga University of Medical Science. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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