Document Detail


Sevoflurane anesthesia in pregnant mice induces neurotoxicity in fetal and offspring mice.
MedLine Citation:
PMID:  23314109     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Each year, over 75,000 pregnant women in the United States undergo anesthesia care. The authors set out to assess the effects of the anesthetic sevoflurane on neurotoxicity in pregnant mice and on learning and memory in fetal and offspring mice.
METHODS: Pregnant mice (gestational day 14) and mouse primary neurons were treated with 2.5% sevoflurane for 2 h and 4.1% sevoflurane for 6 h, respectively. Brain tissues of both fetal and offspring mice (P31) and the primary neurons were harvested and subjected to Western blot and immunohistochemistry to assess interleukin-6, the synaptic markers postsynaptic density-95 and synaptophysin, and caspase-3 levels. Separately, learning and memory function in the offspring mice was determined in the Morris water maze.
RESULTS: Sevoflurane anesthesia in pregnant mice induced caspase-3 activation, increased interleukin-6 levels (256 ± 50.98% [mean ± SD] vs. 100 ± 54.12%, P = 0.026), and reduced postsynaptic density-95 (61 ± 13.53% vs. 100 ± 10.08%, P = 0.036) and synaptophysin levels in fetal and offspring mice. The sevoflurane anesthesia impaired learning and memory in offspring mice at P31. Moreover, interleukin-6 antibody mitigated the sevoflurane-induced reduction in postsynaptic density-95 levels in the neurons. Finally, environmental enrichment attenuated the sevoflurane-induced increases in interleukin-6 levels, reductions of synapse markers, and learning and memory impairment.
CONCLUSIONS: These results suggest that sevoflurane may induce detrimental effects in fetal and offspring mice, which can be mitigated by environmental enrichment. These findings should promote more studies to determine the neurotoxicity of anesthesia in the developing brain.
Authors:
Hui Zheng; Yuanlin Dong; Zhipeng Xu; Gregory Crosby; Deborah J Culley; Yiying Zhang; Zhongcong Xie
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anesthesiology     Volume:  118     ISSN:  1528-1175     ISO Abbreviation:  Anesthesiology     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-22     Completed Date:  2013-04-19     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  1300217     Medline TA:  Anesthesiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  516-26     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Anesthetics, Inhalation / toxicity*
Animals
Animals, Newborn
Brain / drug effects,  growth & development,  metabolism
Female
Maze Learning / drug effects,  physiology
Methyl Ethers / toxicity*
Mice
Mice, Inbred C57BL
Pregnancy
Prenatal Exposure Delayed Effects / chemically induced,  metabolism*
Prenatal Injuries / chemically induced,  metabolism*
Random Allocation
Grant Support
ID/Acronym/Agency:
P01NS044155/NS/NINDS NIH HHS; R01 AG041274/AG/NIA NIH HHS; R01 GM088801/GM/NIGMS NIH HHS; R01NS027713/NS/NINDS NIH HHS; R21 AG029856/AG/NIA NIH HHS; R21 AG038994/AG/NIA NIH HHS; R21NS070153/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Anesthetics, Inhalation; 0/Methyl Ethers; 28523-86-6/sevoflurane
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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