| The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum. | |
| | |
MedLine Citation:
|
PMID: 20519696 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid in asymptomatic pregnancies and placental infections. Ureaplasma parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects on the fetus and chorioamnion of chronic ureaplasma infection in amniotic fluid are dependent on the serovar, dose, and variation of the ureaplasma multiple-banded antigen (MBA) and mba gene. We injected high- or low-dose U. parvum serovar 3, serovar 6, or vehicle intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and Western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005), and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (five or fewer) within amniotic fluid were associated with severe inflammation, whereas the chorioamnion from animals with nine or more mba variants showed little or no inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes. |
| | |
Authors:
|
Christine L Knox; Samantha J Dando; Ilias Nitsos; Suhas G Kallapur; Alan H Jobe; Diane Payton; Timothy J M Moss; John P Newnham |
Related Documents
:
|
8023266 - Transamniotic fetal feeding: enhancement of growth in a rabbit model of intrauterine gr... 2750826 - Postdate fetal surveillance: is 41 weeks too early? 15301286 - Amniopatch, a repairing technique for premature rupture of amniotic membranes in second... 9806296 - Follistatin and activin a in extra-embryonic coelomic and amniotic fluids and maternal ... 20608796 - Is the caesarean section rate a performance indicator of an obstetric unit? 8023266 - Transamniotic fetal feeding: enhancement of growth in a rabbit model of intrauterine gr... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-02 |
Journal Detail:
|
Title: Biology of reproduction Volume: 83 ISSN: 1529-7268 ISO Abbreviation: Biol. Reprod. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-23 Completed Date: 2010-11-30 Revised Date: 2011-12-05 |
Medline Journal Info:
|
Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
|
Languages: eng Pagination: 415-26 Citation Subset: IM |
Affiliation:
|
Institute of Health and Biomedical Innovation, and School of Life Sciences, Queensland University of Technology, Brisbane, Queensland, Australia. c.knox@qut.edu.au |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amniotic Fluid
/
microbiology* Analysis of Variance Animals Bacterial Proteins / genetics* Blotting, Western Chorioamnionitis / genetics, microbiology* Female Inflammation / genetics, microbiology Kidney / microbiology Leukocyte Count Liver / microbiology Maternal-Fetal Exchange* Placenta / microbiology Polymerase Chain Reaction Pregnancy Random Allocation Severity of Illness Index Sheep Ureaplasma / genetics* Ureaplasma Infections / genetics, microbiology* |
| Grant Support | |
ID/Acronym/Agency:
|
HL-65397/HL/NHLBI NIH HHS; R01 HD057869-04/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/multiple-banded antigen, Ureaplasma |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of genetic captive-breeding protocols on sperm quality and fertility in the white-footed mou...
Next Document: CYP3AP1*3 Allele Is Associated With Lipid-Lowering Efficacy of Simvastatin and Atorvastatin in Chine...