Document Detail


The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum.
MedLine Citation:
PMID:  20519696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid in asymptomatic pregnancies and placental infections. Ureaplasma parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects on the fetus and chorioamnion of chronic ureaplasma infection in amniotic fluid are dependent on the serovar, dose, and variation of the ureaplasma multiple-banded antigen (MBA) and mba gene. We injected high- or low-dose U. parvum serovar 3, serovar 6, or vehicle intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and Western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005), and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (five or fewer) within amniotic fluid were associated with severe inflammation, whereas the chorioamnion from animals with nine or more mba variants showed little or no inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes.
Authors:
Christine L Knox; Samantha J Dando; Ilias Nitsos; Suhas G Kallapur; Alan H Jobe; Diane Payton; Timothy J M Moss; John P Newnham
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-02
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-11-30     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  415-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / microbiology*
Analysis of Variance
Animals
Bacterial Proteins / genetics*
Blotting, Western
Chorioamnionitis / genetics,  microbiology*
Female
Inflammation / genetics,  microbiology
Kidney / microbiology
Leukocyte Count
Liver / microbiology
Maternal-Fetal Exchange*
Placenta / microbiology
Polymerase Chain Reaction
Pregnancy
Random Allocation
Severity of Illness Index
Sheep
Ureaplasma / genetics*
Ureaplasma Infections / genetics,  microbiology*
Grant Support
ID/Acronym/Agency:
HL-65397/HL/NHLBI NIH HHS; R01 HD057869/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/multiple-banded antigen, Ureaplasma
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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