Document Detail


Severe neutrophil depletion by leucocyte filters or cytotoxic drug does not improve recovery of contractile function in stunned porcine myocardium.
MedLine Citation:
PMID:  8348569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The contribution of neutrophils to myocardial injury during stunning remains controversial because of conflicting results in neutropenic animals. The goal of this study was to compare the recovery of function in stunned myocardium using two distinct methods for inducing neutropenia in pigs. METHODS: Three groups of pigs were studied: control (n = 6), and made neutropenic by either Leukopak blood filters (n = 7), or cyclophosphamide (n = 7, 50 mg.kg-1 intravenously 4 d prior to study). In anaesthetised open chest pigs, with the heart paced at 110 beats.min-1, the left anterior descending coronary artery was perfused with an extracorporeal circuit at controlled coronary pressure (CP) and the regional coronary blood flow was measured. Systolic wall thickening was determined by sonomicrometry in the left anterior descending and circumflex coronary artery regions. The protocol consisted of 15 min of low flow ischaemia (CP = 40 mm Hg), followed by a staged reperfusion over 10 min back to baseline (CP = 90 mm Hg), and continued for 2 h. Blood filtration was initiated prior to ischaemia and stopped after 90 min reperfusion. RESULTS: In both treated groups during ischaemia and the initial 60 min of reperfusion the neutrophil count was severely depleted to < 5% compared to the control group. Aortic pressure, coronary blood flow during ischaemia, area at risk, and systolic wall thickening in the circumflex region were similar between groups. Recovery of systolic wall thickening in the left anterior descending region after reperfusion was equivalent in all three groups. In the filter group, arrhythmias during ischaemia and reperfusion were significantly less. CONCLUSION: As assessed in the pig model of myocardial stunning and using two different methods, severe neutropenia does not reduce the depression of contractile function.
Authors:
C F Juneau; B R Ito; U del Balzo; R L Engler
Related Documents :
18503209 - Relationship of admission qrs duration and changes in qrs duration with myocardial repe...
8371169 - Temporary focal cerebral ischemia in spontaneously hypertensive rats: the effect of ibu...
10624969 - Sevoflurane and isoflurane do not enhance the pre- and postischemic eicosanoid producti...
10374029 - Efficacy of contraction uncoupling by 2,3-butanedione monoxime during initial reperfusi...
6955529 - Mechanism of mitochondrial damage after coronary reperfusion.
10232769 - Effect of superoxide dismutase and acidified sodium nitrite on infarct size following i...
12540519 - Attenuation of increased myocardial ischaemia-reperfusion injury conferred by hyperchol...
11719159 - Preliminary results on the prediction of countershock success with fibrillation power.
18442919 - Effect of local annular interventions on annular and left ventricular geometry.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cardiovascular research     Volume:  27     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-09-13     Completed Date:  1993-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  720-7     Citation Subset:  IM    
Affiliation:
Department of Veterans Affairs, Medical Center, Research Service, La Jolla, CA 92161.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cyclophosphamide / pharmacology
Disease Models, Animal
Female
Leukocyte Count
Male
Micropore Filters
Myocardial Contraction / physiology*
Myocardial Reperfusion Injury / etiology*,  pathology
Neutropenia / chemically induced
Neutrophils / pathology,  physiology*
Swine
Chemical
Reg. No./Substance:
50-18-0/Cyclophosphamide
Comments/Corrections
Comment In:
Cardiovasc Res. 1993 May;27(5):728-30   [PMID:  8348570 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Endogenous chemical mediators of ventricular arrhythmias in ischaemic heart disease.
Next Document:  Regulation of intrarenal and circulating renin-angiotensin systems in severe heart failure in the ra...