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Severe eosinophilic syndrome associated with the use of probiotic supplements: a new entity?
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MedLine Citation:
PMID:  23259129     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Severe eosinophilic syndromes related to the administration or use of unsuspected immunogenic substances have been described previously. Many of these diseases presented initially as clusters or isolated cases. The spanish toxic oil syndrome, the eosinophilia myalgia syndrome, and nephrogenic systemic fibrosis are examples of such diseases. We describe 2 cases of a severe eosinophilic syndrome characterized by marked peripheral blood eosinophilia (>15,000 cells/ml), mononeuritis multiplex, and necrotizing vasculitis which developed in a close temporal association with the recent onset use of nonprescription probiotics. There was no history of a prior autoimmune disease. Although both cases had prompt response to immunosuppression with rapid resolution of peripheral blood eosinophilia and accompanying constitutional symptoms, they remained with permanent neurological deficits.
Authors:
Fabian A Mendoza; Shivani Purohit; Lawrence Kenyon; Sergio A Jimenez
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Publication Detail:
Type:  Journal Article     Date:  2012-12-06
Journal Detail:
Title:  Case reports in rheumatology     Volume:  2012     ISSN:  2090-6897     ISO Abbreviation:  Case Rep Rheumatol     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-21     Completed Date:  2012-12-24     Revised Date:  2013-04-15    
Medline Journal Info:
Nlm Unique ID:  101585353     Medline TA:  Case Rep Rheumatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  934324     Citation Subset:  -    
Affiliation:
Jefferson Institute of Molecular Medicine and Scleroderma Center, Thomas Jefferson University, Philadelphia, PA 19107, USA ; Rheumatology Division, Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.
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Journal Information
Journal ID (nlm-ta): Case Report Rheumatol
Journal ID (iso-abbrev): Case Report Rheumatol
Journal ID (publisher-id): CRIM.RHEUMATOLOGY
ISSN: 2090-6889
ISSN: 2090-6897
Publisher: Hindawi Publishing Corporation
Article Information
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Copyright © 2012 Fabian A. Mendoza et al.
open-access:
Received Day: 12 Month: 10 Year: 2012
Accepted Day: 4 Month: 11 Year: 2012
Print publication date: Year: 2012
Electronic publication date: Day: 6 Month: 12 Year: 2012
Volume: 2012E-location ID: 934324
PubMed Id: 23259129
ID: 3521401
DOI: 10.1155/2012/934324

Severe Eosinophilic Syndrome Associated with the Use of Probiotic Supplements: A New Entity?
Fabian A. Mendoza12*
Shivani Purohit2
Lawrence Kenyon3
Sergio A. Jimenez1
1Jefferson Institute of Molecular Medicine and Scleroderma Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
2Rheumatology Division, Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
3Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
Correspondence: *Fabian A. Mendoza: fabian.mendoza@jefferson.edu
[other] Academic Editors: K. P. Makaritsis, S. C. Plastiras, and P. E. Prete

1. Introduction

Probiotics are defined by the World Health Organization/Food and Agriculture Organization (WHO/FAO) as live microorganisms that when administered at appropriate doses confer a health benefit to the host by regulation of the gastrointestinal flora [1, 2]. Most frequently, these include Lactobacillus or Bifidobacterium species as well as a range of lactic acid producing bacteria. Being considered a dietary supplement in the USA, the Food and Drug Administration (FDA) regulation of probiotics is not as strict as that for prescription drugs [3]. On the other hand, there is an increased trend in the use of over-the-counter probiotics in patients with rheumatic diseases based on the belief that their use can be beneficial for regulating the immune response beyond the gastrointestinal tract. Whereas the evidence for these claims is marginal at best, there have been numerous reports of adverse events following probiotic use including bacteremia with Lactobacilli, Saccharomyces Boulardii fungemia and endocarditis in postoperative patients, as well as Saccharomyces Cerevisiae fungemia in immunocompromised patients [46]. This report describes two patients who developed a severe eosinophilic syndrome with vasculitis and mononeuritis multiplex, temporally associated with probiotic use. Both patients recalled using a new brand of probiotic between 2 and 4 weeks prior to symptom onset.


2. Case Reports

Case 1

A 55-year-old female with no significant past medical history, presented with sudden onset of arthralgias, myalgias, malaise, night sweats, lower extremity weakness, and paresthesias of 1-week duration. Physical and neuromuscular examination revealed bilateral moderate-to-severe lower extremity weakness, asymmetrical in severity and predominantly distal with right foot drop. She was found to have leukocytosis (29,700 cells/mL) with 56% eosinophils (16,632 cells/mL). Electromyography (EMG) and nerve conduction studies revealed mononeuritis multiplex. Right quadriceps muscle and right sural nerve biopsies performed before the initiation of IV steroids or cyclophosphamide showed severe necrotizing eosinophilic vasculitis (Figure 1). The sural nerve biopsy showed secondary axonal degeneration. Examination of bone marrow demonstrated mild eosinophilia consistent with a reactive process without evidence of malignancy. Flow cytometry of peripheral blood cells was negative for phenotypically abnormal cells. She was treated with IV corticosteroid pulses (methylprednisolone 1 g IV for 3 consecutive days) followed by oral steroids (prednisone 1 mg/Kg/d) with slow titration downward over 10 months and cyclophosphamide 1000 mg/m²/month for 6 months, followed by azathioprine 2 mg/kg/d after the initial 6 months.

Case 2

A 65-year-old female with a history of inactive mild episodic asthma, sinusitis, and a remote history of Hashimoto's thyroiditis presented with sudden onset of abdominal pain, left arm weakness, numbness, right foot drop, diplopia, and profound general malaise. Physical and neuromuscular examination revealed mild left arm weakness along with profound distal right lower extremity weakness and foot drop. No skin lesions were noticed. Laboratory studies showed leukocytosis of 24,300 cells/mL with severe eosinophilia of 76% (18,468 cells/mL). EMG and nerve conduction studies showed evidence of mononeuritis multiplex and in addition, a mild underlying diffuse peripheral neuropathy. A right quadriceps muscle biopsy performed 1 week after initiation of IV steroids showed necrotizing vasculitis without eosinophils (Figure 2). By the time the biopsy was performed, the peripheral blood eosinophilia had resolved and apparently also the eosinophilic component of the vasculitis. A right sural nerve biopsy performed at the same time demonstrated a moderate chronic axonal neuropathy with numerous degenerating myelinated and unmyelinated fibers but no evidence of vasculitis. Bone marrow studies demonstrated 45% eosinophils without evidence of malignancy or atypia. She was treated with the same initial therapeutic regimen as Case 1 which included IV corticosteroids and oral cyclophosphamide, followed by mycophenolate mofetil (MMF) 2 grams/day.

Extensive questioning did not disclose the use of any other over-the-counter products, including L-tryptophan, recent use of leukotriene inhibitors, or any other new medication. However, both patients recalled the use of a new brand of probiotics described as an “extra-strength concentration” in a boxed blister pack, purchased over-the-counter in a Philadelphia metropolitan area pharmacy. Unfortunately we were unable to unequivocally identify the specific brand. None of the patients had gastrointestinal complaints, but they used the probiotic to “improve their immune system”.

Both patients were negative for FIP-1 (PDGF) mutations which are commonly present in myeloproliferative forms of eosinophilic syndrome. Furthermore, there were no abnormalities detected by peripheral blood or bone marrow flow cytometry, nor abnormal PDGF-related translocations or other cytogenetic abnormalities. None of the patients developed respiratory symptoms or had pulmonary infiltrates on high-resolution chest-computed tomography scans.

Response to IV corticosteroids followed by immunosuppressive therapy was optimal in both cases, with rapid resolution of peripheral blood eosinophilia and of the generalized symptoms. Although there was some degree of neurological improvement, to date, both patients have remained with permanent motor and sensory deficits in the lower extremities requiring orthotic devices for ambulation.


3. Discussion

We describe here the clinical manifestations and extensive laboratory, histopathologic, and radiological studies of two patients who developed an abrupt onset of a severe eosinophilic syndrome with prominent constitutional symptoms, necrotizing vasculitis, and mononeuritis multiplex, occurring in close temporal association with the ingestion of a probiotic supplement. Both of the patients were initially diagnosed with Churg-Strauss vasculitis, and one of them fulfilled the American College of Rheumatology diagnostic criteria proposed for Churg-Strauss [7]. There was histological evidence of an eosinophilic necrotizing vasculitis in one of the cases, whereas the second case showed vasculitis without tissue eosinophils. It is very likely that the absence of eosinophils in the tissue vasculitic infiltrate in the second case is related to the IV corticosteroid therapy which was instituted prior to the biopsy. However, a more detailed analysis taking into consideration the severity and sudden onset of their general symptoms, absence of prior history of asthma in one of the patients (and nonactive mild episodic asthma in the other), absence of lung parenchymal involvement in both patients, negative ANCA (immunofluorescence and ELISA MPO/PR3 antibodies), marked eosinophilia (above 50% of the total WBC in both patients), and the close temporal association with consumption of a probiotic agent leads us to conclude that their disease was induced by the ingestion of a foreign agent presenting as an eosinophilic syndrome. Thus, we believe that these two cases may represent a novel eosinophilic clinical entity distinct from Churg-Strauss syndrome.

The cases presented differ from eosinophilia myalgia syndrome (EMS) because of the absence of skin edema or fibrosis, the lack of a history of L-tryptophan ingestion, and the presence of acute neurological involvement as opposed to the subacute or chronic course of neurological manifestations typically described in EMS [8].

Numerous severe eosinophilic syndromes related to unsuspected immunogenic substances have been well described in the past presenting initially as either clusters or isolated cases which subsequently reached epidemic proportions. The Spanish toxic oil and EMS are good examples [8, 9]. Although it was generally accepted that the EMS epidemic had disappeared following the withdrawal of the contaminated L-tryptophan containing preparations, a nonepidemic case of EMS has very recently been reported [10]. In both disorders, the initial presentation of isolated or clustered cases associated with either a nonregulated contaminated food or dietary supplement delayed the recognition of the etiological agent and the prevention of new cases [11]. Both of the patients described here presented to medical attention in the same month, and apparently both acquired the suspected agent at approximately the same time raising epidemiological concerns of additional unreported or undiagnosed cases.

Prior experience in unveiling the etiology of the eosinophilic syndromes has shown that a high index of suspicion is required. The description of these two cases may allow the recognition of similar cases and remind the medical community that the widespread use of agents seemingly considered to be beneficial and free of side effects may occasionally have serious consequences. In this paper, we welcome government efforts focused on the establishment and enforcement of regulations promoting “good manufacturing practices” for dietary supplements.


References
1. Food and Agriculture Organization of the United Nations and World Health OrganizationGuidelines for the evaluation of probiotics in foodReport of a Joint FAO/WHO Working GroupYear: 2002Ontario, CanadaFood and Agriculture Organization of the United Nations and World Health Organization
2. Guandalini S. Probiotics for prevention and treatment of diarrheaJournal of Clinical GastroenterologyYear: 201145S149S15321992955
3. Hoffman FA,Heimbach JT,Sanders ME,Hibberd PL. Executive summary: scientific and regulatory challenges of development of probiotics as foods and drugsClinical Infectious DiseasesYear: 200846supplement 2S53S572-s2.0-4004910374118181723
4. Soleman N,Laferl H,Kneifel W,et al. How safe is safe?—a case of Lactobacillus paracasei ssp. paracasei endocarditis and discussion of the safety of lactic acid bacteriaScandinavian Journal of Infectious DiseasesYear: 200335107597622-s2.0-024238315814606618
5. Muñoz P,Bouza E,Cuenca-Estrella M,et al. Saccharomyces cerevisiae fungemia: an emerging infectious diseaseClinical Infectious DiseasesYear: 20054011162516342-s2.0-1934437675015889360
6. Mackay AD,Taylor MB,Kibbler CC,Hamilton-Miller JMT. Lactobacillus endocarditis caused by a probiotic organismClinical Microbiology and InfectionYear: 1999552902922-s2.0-003297228211856270
7. Masi AT,Hunder GG,Lie JT,et al. The American College of Rheumatology 1990 Criteria for the classification of Churg-Strauss syndrome (allergic granulomatosis and angiitis)Arthritis and RheumatismYear: 1990338109411002-s2.0-00250913912202307
8. Silver RM,Heyes MP,Maize JC,Quearry B,Vionnet-Fuasset M,Sternberg EM. Scleroderma, fasciitis, and eosinophilia associated with the ingestion of tryptophanThe New England Journal of MedicineYear: 1990322138748812-s2.0-00253075791690352
9. Alonso-Ruiz A,Calabozo M,Perez-Ruiz F,Mancebo L. Toxic oil syndrome. A long-term follow-up of a cohort of 332 patientsMedicineYear: 19937252852952-s2.0-00275013098412642
10. Allen JA,Peterson A,Sufit R,et al. Post-epidemic eosinophilia-myalgia syndrome associated with L-tryptophanArthritis & RheumatismYear: 2011633633363921702023
11. Sullivan EA,Staehling N,Philen RM. Eosinophilia-myalgia syndrome among the non-L-tryptophan users and pre-epidemic casesJournal of RheumatologyYear: 19962310178417872-s2.0-00298252618895159

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