Document Detail


Severe dermatophytosis due to Trichophyton mentagrophytes var. interdigitale in flocks of green iguanas (Iguana iguana).
MedLine Citation:
PMID:  22533321     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Objective: To describe the clinical, mycological, histopathological and molecular findings in green iguanas (Iguana iguana) affected with severe dermatophytosis in selected flocks near Tehran, Iran. Materials and Methods: Samples were collected from the scales of skin lesions and tested with standard mycological methods and dermatophyte-specific PCR amplification analysis using the primer pair for the chitin synthase 1(CHS1) gene. Results: All iguanas were definitively diagnosed with dermatophytosis using both traditional and molecular diagnostic methods. PCR fingerprinting profiles using simple repetitive (GACA)4 primers showed that all diagnosed iguanas had the same pattern profile. Intraspecific variability was not observed for these isolates. Oligonucleotide sequencing of CHS1 gene PCR products confirmed Trichophyton mentagrophytes var. interdigitale as the infectious agent. Clinical Significance: These results suggest that (GACA)4-based PCR has utility both as a simple and rapid method for identification of dermatophyte species and for differentiation of T. mentagrophytes variants.
Authors:
A R Khosravi; H Shokri; A Rostami; I A Tamai; A Erfanmanesh; I Memarian
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of small animal practice     Volume:  53     ISSN:  1748-5827     ISO Abbreviation:  J Small Anim Pract     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0165053     Medline TA:  J Small Anim Pract     Country:  England    
Other Details:
Languages:  eng     Pagination:  286-91     Citation Subset:  IM    
Copyright Information:
© 2012 British Small Animal Veterinary Association.
Affiliation:
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Faculty of Veterinary Medicine, University of Mazandaran, Amol, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Academic Center of Education, Culture and Research (ACECR), Tehran, Iran.
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