| Severe acute respiratory syndrome coronavirus 3C-like protease-induced apoptosis. | |
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MedLine Citation:
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PMID: 16553810 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is an important issue for the treatment and prevention of severe acute respiratory syndrome. Recently, SARS-CoV has been demonstrated to induce cell apoptosis in Vero-E6 cells. The possible role of SARS-CoV 3C-like protease (3CLpro) in virus-induced apoptosis is characterized in this study. Growth arrest and apoptosis via caspase-3 and caspase-9 activities were demonstrated in SARS-CoV 3CLpro -expressing human promonocyte cells. The fluorescence intensity of dihydrorhodamine 123 staining indicated that cellular reactive oxygen species were markedly increased in SARS-CoV 3CLpro -expressing cells. Moreover, in vivo signalling pathway assay indicated that 3CLpro increased the activation of the nuclear factor-kappa B-dependent reporter, but inhibited activator protein-1-dependent transcription. This finding is likely to be responsible for virus-induced apoptotic signalling. |
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Authors:
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Cheng-Wen Lin; Kuan-Hsun Lin; Tsung-Han Hsieh; Shi-Yi Shiu; Jeng-Yi Li |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS immunology and medical microbiology Volume: 46 ISSN: 0928-8244 ISO Abbreviation: FEMS Immunol. Med. Microbiol. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-23 Completed Date: 2006-06-20 Revised Date: 2007-10-11 |
Medline Journal Info:
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Nlm Unique ID: 9315554 Medline TA: FEMS Immunol Med Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 375-80 Citation Subset: IM |
Affiliation:
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Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan. cwlin@mail.cmu.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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chemistry Apoptosis / immunology* Caspase 3 Caspase 9 Caspases / metabolism Cell Line Cysteine Endopeptidases / biosynthesis, genetics, immunology* Humans Microscopy, Fluorescence NF-kappa B / immunology Reactive Oxygen Species / metabolism Rhodamines / chemistry SARS Virus / enzymology*, genetics, immunology Severe Acute Respiratory Syndrome / virology Signal Transduction Transcription Factor AP-1 / immunology Transfection Viral Proteins / biosynthesis, genetics, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/NF-kappa B; 0/Reactive Oxygen Species; 0/Rhodamines; 0/Transcription Factor AP-1; 0/Viral Proteins; 109244-58-8/dihydrorhodamine 123; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.28/3C proteases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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