| Severe In vivo hyper-homocysteinemia is not associatedwith elevation of amyloid-beta peptides in the Tg2576 mice. | |
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MedLine Citation:
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PMID: 20555139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since hyper-homocysteinemia (HHcy) was recognized as a risk factor for Alzheimer's disease (AD), many studies tried to induce HHcy in animal models to investigate its effect on amyloid-beta protein precursor (AbetaPP) metabolism. Previous reports found that HHcy induced in AD transgenic mouse models, by either feedina a methionine-enriched diet or vitamin Bs deficient diet, is associated with elevation of amyloid-beta (Abeta) levels. However, there is no data available on the effect of dietary intervention which combines both excessive methionine and low levels of vitamin Bs on amyloidogenesis in any of these models. In the current study, we investigated the effect of a combination diet, which was both enriched in methionine and deficient in folate, vitamin B6 and B12, in an AD mouse model, the Tg2576. We found that 7 months treatment of this diet induced severe HHcy in these mice with plasma homocysteine level higher than 150 microM. However, no difference was detected in brain Abeta levels or deposition between the diet-treated and control group. As shown by western blot, severe HHcy did not alter the steady state levels of proteins involved in AbetaPP metabolism, either. These results demonstrate that this combination diet-induced severe HHcy does not influence amyloidogenesis in vivo. |
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Authors:
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Jia-Min Zhuo; Domenico Praticò |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Alzheimer's disease : JAD Volume: 21 ISSN: 1875-8908 ISO Abbreviation: J. Alzheimers Dis. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-11-02 Revised Date: 2011-02-23 |
Medline Journal Info:
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Nlm Unique ID: 9814863 Medline TA: J Alzheimers Dis Country: Netherlands |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alzheimer Disease
/
etiology*,
genetics,
metabolism*,
pathology Amyloid beta-Peptides / metabolism* Amyloid beta-Protein Precursor / genetics Animals Cerebral Cortex / metabolism Disease Models, Animal Enzyme-Linked Immunosorbent Assay / methods Gene Expression Regulation / genetics Hippocampus / metabolism Humans Hyperhomocysteinemia / chemically induced, complications*, pathology Methionine / administration & dosage Mice Mice, Transgenic Peptide Fragments / metabolism* Random Allocation Vitamin B 12 / administration & dosage |
| Grant Support | |
ID/Acronym/Agency:
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AG-22512/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Peptides; 0/Amyloid beta-Protein Precursor; 0/Peptide Fragments; 0/amyloid beta-protein (1-40); 0/amyloid beta-protein (1-42); 63-68-3/Methionine; 68-19-9/Vitamin B 12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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