Document Detail


Severe heat shock induces nucleolar accumulation of mRNAs in Trypanosoma cruzi.
MedLine Citation:
PMID:  22952745     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several lines of evidence have shown that, besides its traditional function in ribosome biogenesis, the nucleolus is also involved in regulating other cellular processes such as mRNA metabolism, and that it also plays an important role as a sensor and coordinator of the stress response. We have recently shown that a subset of RNA Binding Proteins and the poly(A)+ RNA are accumulated into the Trypanosoma cruzi nucleolus after inducing transcription inhibition with Actinomycin D. In this study, we investigated the behaviour of the T. cruzi mRNA population in parasites subjected to severe heat shock, an environmental stress that also decreases the rate of RNA synthesis. We found that the bulk of poly(A)+ RNA is reversibly accumulated into the nucleolus when exposing T. cruzi epimastigote forms to severe heat shock. However, the Hsp70 mRNA was able to bypass such nucleolar accumulation. Together, these data reinforce the idea about the involvement of the T. cruzi nucleolus in mRNA metabolism during an environmental stress response. Interestingly, T. brucei procyclic forms did not induce nucleolar accumulation of poly(A)+ RNA under such stress condition, suggesting that different trypanosomatids have adopted different responses to deal with the same stress conditions.
Authors:
Ezequiel Názer; Ramiro E Verdún; Daniel O Sánchez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-27
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-06     Completed Date:  2013-02-19     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e43715     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Biotecnológicas - UNSAM-CONICET, San Martín, Provincia de Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Animals
Cell Nucleolus / metabolism*
HSP70 Heat-Shock Proteins / genetics
Heat-Shock Response*
Poly A / metabolism
Protozoan Proteins / genetics
RNA, Messenger / metabolism
Trypanosoma cruzi / cytology*,  genetics,  metabolism,  physiology*
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Protozoan Proteins; 0/RNA, Messenger; 24937-83-5/Poly A
Comments/Corrections

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