Document Detail

Severe heat shock induces nucleolar accumulation of mRNAs in Trypanosoma cruzi.
MedLine Citation:
PMID:  22952745     Owner:  NLM     Status:  MEDLINE    
Several lines of evidence have shown that, besides its traditional function in ribosome biogenesis, the nucleolus is also involved in regulating other cellular processes such as mRNA metabolism, and that it also plays an important role as a sensor and coordinator of the stress response. We have recently shown that a subset of RNA Binding Proteins and the poly(A)+ RNA are accumulated into the Trypanosoma cruzi nucleolus after inducing transcription inhibition with Actinomycin D. In this study, we investigated the behaviour of the T. cruzi mRNA population in parasites subjected to severe heat shock, an environmental stress that also decreases the rate of RNA synthesis. We found that the bulk of poly(A)+ RNA is reversibly accumulated into the nucleolus when exposing T. cruzi epimastigote forms to severe heat shock. However, the Hsp70 mRNA was able to bypass such nucleolar accumulation. Together, these data reinforce the idea about the involvement of the T. cruzi nucleolus in mRNA metabolism during an environmental stress response. Interestingly, T. brucei procyclic forms did not induce nucleolar accumulation of poly(A)+ RNA under such stress condition, suggesting that different trypanosomatids have adopted different responses to deal with the same stress conditions.
Ezequiel Názer; Ramiro E Verdún; Daniel O Sánchez
Related Documents :
7826375 - Cloning and sequencing of the cdna for the light subunit of human liver gamma-glutamylc...
24958385 - Regulation systems of bacteria such as escherichia coli in response to nutrient limitat...
8208605 - Two human homologues of saccharomyces cerevisiae swi2/snf2 and drosophila brahma are tr...
22684895 - Microrna-590-5p regulates proliferation and invasion in human hepatocellular carcinoma ...
22470405 - Regulation of mirna 219 and mirna clusters 338 and 17-92 in oligodendrocytes.
20970745 - Regulation of u6 promoter activity by transcriptional interference in viral vector-base...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-27
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-09-06     Completed Date:  2013-02-19     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e43715     Citation Subset:  IM    
Instituto de Investigaciones Biotecnológicas - UNSAM-CONICET, San Martín, Provincia de Buenos Aires, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Active Transport, Cell Nucleus
Cell Nucleolus / metabolism*
HSP70 Heat-Shock Proteins / genetics
Heat-Shock Response*
Poly A / metabolism
Protozoan Proteins / genetics
RNA, Messenger / metabolism
Trypanosoma cruzi / cytology*,  genetics,  metabolism,  physiology*
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Protozoan Proteins; 0/RNA, Messenger; 24937-83-5/Poly A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Global projections of 21st century land-use changes in regions adjacent to Protected Areas.
Next Document:  Genetic diversity and population structure of Indian golden silkmoth (Antheraea assama).