Document Detail

Seven-day storage of single donor platelets in polyolefin bags: clinical, biochemical, morphological and microbiological evaluation.
MedLine Citation:
PMID:  3360514     Owner:  NLM     Status:  MEDLINE    
We compared the in vitro and in vivo function of fresh and stored platelet concentrates (PCs) collected by an automated continuous-flow blood cell separator (CS 3000 Fenwal) in a closed-system apheresis kit in order to evaluate the possibility of extending the storage time to seven days with the polyolefin container (PL-732). The initial 220 ml platelet volume (5.14 +/- 1.23 x 10(11) was divided into two parts. Half was transfused and the other half was stored for 7 days. All cultured units were negative for bacterial contamination. Mean counts for fresh and stored platelets were respectively 2.34 +/- 0.59 and 2.17 +/- 0.50 X 10(11)/100 ml of PCs (mean recovery 88.7 +/- 11.9%). The pO2 levels were maintained during storage (179.9 +/- 30.5 mmHg) but pCO2, pH, LDH, osmolality, glucose consumption, bicarbonates, ATP, and osmotic stress values changed significantly after 7 days storage. From a clinical point of view, in 14 patients receiving a total of 38 PC transfusions no statistically significant change in corrected post-transfusional levels was observed between fresh and stored PC. Biochemical and morphological data and clinical results suggest that PCs collected with CS-3000 blood cell separator in a closed system and stored for 7 days in polyolefin bags (PL-732) can be satisfactorily employed in clinical practice.
A Dragani; A Iacone; A Angelini; D D'Antonio; P Accorsi; G Fioritoni; L Geraci; A Berardi; A Quaglietta; P Di Bartolomeo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of artificial organs     Volume:  11     ISSN:  0391-3988     ISO Abbreviation:  Int J Artif Organs     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-05-31     Completed Date:  1988-05-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7802649     Medline TA:  Int J Artif Organs     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  51-6     Citation Subset:  IM    
Institute of Hematology, Division of Hematology and Blood Bank, Pescara, Italy.
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MeSH Terms
Blood Platelets* / microbiology,  ultrastructure
Blood Preservation*
Blood Transfusion
Platelet Count
Platelet Function Tests
Platelet Transfusion
Time Factors
Reg. No./Substance:
0/Plastics; 0/Polyenes; 83136-87-2/PL 732

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