Document Detail


Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data.
MedLine Citation:
PMID:  23203297     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT. Owing to the limited source of human skin (HS) and the ethical restrictions of using animals in experiments, in vitro skin equivalents are a possible alternative for conducting particle penetration experiments. The conditions for conducting penetration experiments with model particles, 15-nm gold nanoparticles (AuNP), through nonsealed skin equivalents are described for the first time. These conditions include experimental setup, sterility conditions, effective applied dose determination, skin sectioning, and skin integrity check. Penetration at different exposure times (two and 24 h) and after tissue fixation (fixed versus unfixed skin) are examined to establish a benchmark in comparison to HS in an attempt to get similar results to HS experiments presented earlier. Multiphoton microscopy is used to detect gold luminescence in skin sections. λex=800  nm is used for excitation of AuNP and skin samples, allowing us to determine a relative index for particle penetration. Despite the observed overpredictability of penetration into skin equivalents, they could serve as a first fast screen for testing the behavior of nanoparticles and extrapolate their penetration behavior into HS. Further investigations are required to test a wide range of particles of different physicochemical properties to validate the skin equivalent-human skin particle penetration relationship.
Authors:
Hagar I Labouta; Sibylle Thude; Marc Schneider
Related Documents :
17116417 - Report of the iwgt working group on strategies and interpretation of regulatory in vivo...
3390507 - Effects of treatment-induced mortality and tumor-induced mortality on tests for carcino...
6337827 - Comparison between carcinogenicity and mutagenicity based on chemicals evaluated in the...
7179307 - A rapid procedure to efficiently clean blenders repeatedly used to prepare dosed feed f...
11827567 - Alternatives to animal testing in the safety evaluation of products.
15382187 - Expanded paper towel test: an objective test of urine loss for stress incontinence.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2012-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  61218     Citation Subset:  IM    
Affiliation:
Helmholtz Center for Infection Research, Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, GermanycUniversity of Alexandria, Department of Pharmaceutics, Alexandria, Egypt.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Polymer reinforcement using liquid-exfoliated boron nitride nanosheets.
Next Document:  Colistin resistance in gram-negative bacteria during prophylactic topical colistin use in intensive ...