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Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides and disease control by ifn-a and puva therapy.
MedLine Citation:
PMID:  22233400     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background:  Neoplastic T cells recruitment into the skin is a critical step in mycosis fungoides (MF) pathogenesis and the cutaneous T-cell attracting chemokine CTACK/CCL27 might be involved. Objectives:  Aim of our study was to investigate the clinical and prognostic significance of CTACK/CCL27 levels in patients with early stage MF. Methods:  Serum samples and skin biopsy specimens were collected from 15 patients at the time of the diagnosis, and after the end of treatment with PUVA/IFNα2b combination therapy. Serum samples were also collected from 20 healthy donors as controls. CTACK/CCL27 serum levels were analyzed by ELISA assays. CTACK/CCL27 tissue expression was determined by immunohistochemistry on skin biopsy specimens taken at diagnosis and after therapy. Event-free survival was taken as primary clinical outcome. All statistical analyses were performed using the SPSS statistical package. Results:  In MF patients at diagnosis CTACK/CCL27 serum levels were not significantly different from healthy controls, whereas CTACK/CCL27 expression in skin was increased in 87% of cases compared to normal controls. After therapy all patients obtained a clinical complete remission, serum levels did not change significantly and tissue expression remained abnormal in 80% of patients, even if a complete histological remission was recorded. Serum levels were not significantly different in cases with different intensity of cutaneous immunostaining. Eight patients experienced a relapse: the combination of high CTACK/CCL27 levels both in sera and in skin increased the probability to experience an event at 51 months from 36% to 83%. Conclusions:  Our data seem to indicate that CTACK/CCL27 levels in skin and sera after therapy might be correlated with risk of recurrence.
Authors:
G Goteri; S Rupoli; A Campanati; A Zizzi; P Picardi; M Cardelli; F Giantomassi; L Canafoglia; F Marchegiani; G Mozzicafreddo; G Brandozzi; D Stramazzotti; G Ganzetti; R Lisa; O Simonetti; A Offidani; I Federici; G Filosa; P Leoni
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-11
Journal Detail:
Title:  The British journal of dermatology     Volume:  -     ISSN:  1365-2133     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 British Association of Dermatologists.
Affiliation:
Anatomic Pathology Clinic of Hematology Dermatological Clinic, Ancona Hospital, Polytechnic Marche University Advanced Technology Center for Aging Research, Scientific Technological Area and Scientific Direction Division of Dermatology, I.N.R.C.A- I.R.C.C.S., Ancona Division of Dermatology, Murri Hospital, Jesi, Italy.
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