Document Detail


Serum resistance of Pasteurella multocida in avian and porcine sera, and comparative virulence investigations of selected serum-sensitive and resistant strains in chickens.
MedLine Citation:
PMID:  12396364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Growth in serum of Pasteurella multocida and related species in chicken, turkey, duck and pig sera were compared, and selected serum-resistant and serum-sensitive strains were inoculated into 18-week-old layers. Eighty-seven field strains of Pasteurella spp. and nine reference strains representing different clones defined by restriction endonuclease analysis (REA) profiles were used in the study. Serum activity was measured by changes in the optical density (OD) of the serum after inoculation and incubation at 41 degrees C for chicken, turkey and duck serum and 39 degrees C for pig serum. Serum activity was measured by comparison with previously determined serum-resistant (P-1059) and serum-sensitive (CU vaccine) strains, and classified into highly serum-resistant, moderately serum-resistant and serum-sensitive. Strains of the same REA type were found to have identical growth curves and the same maximum OD values when tested in serum from the same host species. Turkey serum was shown to be less inhibitory to a wide range of P. multocida strains than chicken, duck and pig sera. Serum-resistant strains were demonstrated among avian as well as mammalian strains. Among the avian strains, the proportion of serum-resistant strains was higher in outbreak strains than in strains from apparently healthy carriers. Removal of the capsule from selected strains by hyaluronidase treatment failed to change the serum activity. The most severe lesions in experimentally infected chickens were produced by a serum-resistant strain; however, lesions were also found in chickens infected by serum-sensitive strains, indicating the involvement of multiple factors in the virulence of P. multocida. Further investigations on serum resistance are indicated in order to relate other host and bacterial factors responsible for the development of fowl cholera.
Authors:
Amandus P Muhairwa; Jens P Christensen; Magne Bisgaard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Avian pathology : journal of the W.V.P.A     Volume:  31     ISSN:  0307-9457     ISO Abbreviation:  Avian Pathol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-10-24     Completed Date:  2002-12-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8210638     Medline TA:  Avian Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
Affiliation:
Department of Veterinary Medicine and Public Health, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3021, Chuo Kikuu Morogoro Tanzania. apm@kvl.dk
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickens
Disease Susceptibility / veterinary
Ducks
Immunity, Innate
Pasteurella Infections / blood,  physiopathology,  veterinary*
Pasteurella multocida / pathogenicity,  physiology*
Poultry Diseases / microbiology*
Species Specificity
Swine
Turkeys
Virulence

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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