Document Detail


Serum reserve cholesterol binding capacity (SRCBC): the relative importance of different lipoprotein classes.
MedLine Citation:
PMID:  6794547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Findings by several authors have motivated studies in our laboratory on the relationship between HDL and "serum reserve cholesterol binding capacity" (SRCBC). We found that incubation for 25 hours at 37 degrees C provided optimal conditions for uptake and saturation when 14 mg sonicated and pulverized cholesterol was added to 1 ml serum. Upon separation of serum to which 14C labelled cholesterol had been added, into lipoprotein classes, a higher cpm:protein ratio than expected (from the free cholesterol:protein ratio, assuming isotope exchange exclusively) was found in LDL and the HDL fraction. In a second series of experiments, the different lipoprotein classes were separated prior to addition of 14C-cholesterol. The highest capacity for uptake of labelled cholesterol was found in the HDL fraction. The major part of the 14C-cholesterol appeared in the LDL density area when this HDL fraction was subjected to repeated ultracentrifugation. This suggested that the uptake of added cholesterol by HDL had led to the formation of an altered particle with density characteristics similar to those of LDL particles. This was confirmed by the demonstration of a "new" particle which contained apoA-I, apoA-II, and apoE, but no apoB or Lp(a) antigen. Its electrophoretic mobility was similar to that of HDL. Electron microscopy revealed that this particle is larger than normal HDL. The findings suggest that a sub-population of HDL molecules may be responsible for serum binding of added excess cholesterol.
Authors:
A L Børresen; K Berg
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artery     Volume:  9     ISSN:  0098-6127     ISO Abbreviation:  Artery     Publication Date:  1981  
Date Detail:
Created Date:  1981-12-22     Completed Date:  1981-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7508494     Medline TA:  Artery     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  96-119     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Apolipoprotein A-I
Apolipoprotein A-II
Apolipoproteins / isolation & purification
Cholesterol / blood*
Electrophoresis, Polyacrylamide Gel
Female
Humans
Lipoproteins / blood*
Lipoproteins, HDL / blood,  isolation & purification
Lipoproteins, VLDL / blood,  isolation & purification
Male
Temperature
Time Factors
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoprotein A-II; 0/Apolipoproteins; 0/Lipoproteins; 0/Lipoproteins, HDL; 0/Lipoproteins, VLDL; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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