Document Detail


Serum regulates adipogenesis of mesenchymal stem cells via MEK/ERK-dependent PPARgamma expression and phosphorylation.
MedLine Citation:
PMID:  19243475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mesenchymal stem cells (MSCs) provide us an excellent cellular model to uncover the molecular mechanisms underlying adipogenic differentiation of adult stem cells. PPARgamma had been considered as an important molecular marker of cells undergoing adipogenic differentiation. Here, we demonstrated that expression and phosphorylation of PPARgamma could be found in bone marrow-derived MSCs cultured in expansion medium without any adipogenic additives (dexamethasone, IBMX, insulin or indomethacin). Then, PPARgamma was dephosphorylated in MSCs during the process of adipogenic differentiation. We then found that inhibition of MEK activation by specific inhibitor (PD98059) counteracted the PPARgamma expression and phosphorylation. However, expression and phosphorylation of PPARgamma did not present in MSCs cultured in medium with lower serum concentration. When these MSCs differentiated into adipocytes, no phosphorylation could be detected to accompany the expression of PPARgamma. Moreover, exposure of MSCs to higher concentration of serum induced stronger PPARgamma expression, and subsequently enhanced their adipogenesis. These data suggested that activation of the MEK/ERK signalling pathway by high serum concentration promoted PPARgamma expression and phosphorylation, and subsequently enhanced adipogenic differentiation of MSCs.
Authors:
Ling Wu; Xiaoxiao Cai; Hai Dong; Wei Jing; Yuanding Huang; Xingmei Yang; Yao Wu; Yunfeng Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-20
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  14     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  922-32     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, P. R. China.
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis* / drug effects
Animals
Cells, Cultured
Enzyme Activation / drug effects
Extracellular Signal-Regulated MAP Kinases / metabolism*
Gene Expression Regulation / drug effects
MAP Kinase Signaling System / drug effects
Mesenchymal Stem Cells / cytology*,  drug effects,  enzymology*,  metabolism
Mice
Mice, Inbred BALB C
Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors,  metabolism*
PPAR gamma / metabolism*
Phosphorylation / drug effects
Protein Kinase Inhibitors / pharmacology
Serum / metabolism*
Chemical
Reg. No./Substance:
0/PPAR gamma; 0/Protein Kinase Inhibitors; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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