Document Detail


Serum protein N-glycosylation in paediatric non-alcoholic fatty liver disease.
MedLine Citation:
PMID:  22434757     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: We have previously shown the potential of glycomics to distinguish patients with steatosis from patients with non-alcoholic steatohepatitis (NASH) in an adult population. The pattern of disease in paediatric patients is distinct from adults. The objective of this study was to characterize the N -glycomic profile of children with varying degrees of non-alcoholic fatty liver disease (NAFLD) and identify potential biomarker profiles of disease.
METHODS: Serum protein N-glycosylation patterns of 51 paediatric NAFLD patients were assessed with deoxyribonucleic acid sequencer-assisted fluorophore-assisted capillary electrophoresis and compared with histology.
RESULTS: Peak 1 (NGA2F) is the most significantly elevated N-glycan in paediatric NASH patients with peak 5 (NA2) demonstrating the largest decrease. The logarithmically transformed ratio of peak 1 to peak 5 was -0.85 (standard deviation [SD] 0.22) in patients with steatosis and borderline NASH and -0.73 (SD 0.12) in NASH (P = 0.02). The biomarker correlated well with the amount of lobular inflammation with a consistent increase of marker score in ascending stage of lobular inflammation. There was also a trend in differentiating patients with significant fibrosis ≥F2; -0.74 (SD 0.13) from patients with no/minimal fibrosis <F2; -0.86 (SD 0.24), P = 0.06. Analysis of the N-glycans on immunoglobulin G confirmed the undergalactosylation status typical for chronic inflammatory conditions.
CONCLUSIONS: This study is the first glycomic analysis performed in a paediatric NAFLD population. In agreement with the results obtained in adults, B cells play a dominant role in the N-glycan alterations of paediatric NASH patients.
Authors:
B Blomme; E Fitzpatrick; A Quaglia; R De Bruyne; A Dhawan; H Van Vlierberghe
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Publication Detail:
Type:  Journal Article     Date:  2012-02-10
Journal Detail:
Title:  Pediatric obesity     Volume:  7     ISSN:  2047-6310     ISO Abbreviation:  Pediatr Obes     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101572033     Medline TA:  Pediatr Obes     Country:  England    
Other Details:
Languages:  eng     Pagination:  165-73     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Pediatric Obesity © 2012 International Association for the Study of Obesity.
Affiliation:
Department of Hepatology and Gastroenterology, Ghent University Hospital, Ghent, Belgium.
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