Document Detail

Serum non-coding RNAs as biomarkers for osteoarthritis progression after ACL injury.
MedLine Citation:
PMID:  22944527     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The aim of this study was to examine serum non-coding RNAs as potential biomarkers for cartilage damage associated with anterior cruciate ligament (ACL) injury.
METHODS: Serum was obtained from 80 patients 1 year after surgery for ACL injury and 60 normal donors without overt skeletal injury. Total serum RNA was isolated, small non-coding RNAs profiled by TaqMan array MicroRNA (miRNA) analysis and individual small RNA assays performed by quantitative TaqMan RT-PCR (qPCR). Semi-quantitative magnetic resonance imaging (MRI) analysis was performed using Whole Organ Magnetic Resonance Knee Score (WORMS) scoring for analysis of cartilage damage.
RESULTS: Initial TaqMan array miRNA profiling showed an increased serum concentration of a small nucleolar RNA (snoRNA), U48, in five patients with cartilage damage compared with that in five patients without cartilage damage and six normal donors. Independent qPCR analysis of snoRNAs in serum from all patients and normal donors showed a strong association between the serum level of another snoRNA, U38, and cartilage damage in ACL injury patients and together with snoRNA, U48, clear distinction between ACL injury patients and normal donors.
CONCLUSION: SnoRNAs U38 and U48 are significantly elevated in the serum of patients developing cartilage damage at 1 year after ACL injury. Serum levels of U38 have the potential to facilitate early diagnosis of patients with cartilage damage after ACL injury. This study suggests serum non-coding RNAs may serve as novel noninvasive biomarkers for the detection and assessment of cartilage damage after ACL injury.
L Zhang; M Yang; P Marks; L M White; M Hurtig; Q-S Mi; G Divine; G Gibson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-31
Journal Detail:
Title:  Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society     Volume:  20     ISSN:  1522-9653     ISO Abbreviation:  Osteoarthr. Cartil.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-03-12     Revised Date:  2013-12-05    
Medline Journal Info:
Nlm Unique ID:  9305697     Medline TA:  Osteoarthritis Cartilage     Country:  England    
Other Details:
Languages:  eng     Pagination:  1631-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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MeSH Terms
Anterior Cruciate Ligament / injuries*
Biological Markers / blood
Cartilage, Articular / injuries*,  pathology
Disease Progression
Knee Injuries / blood,  complications*,  diagnosis
Magnetic Resonance Imaging
Middle Aged
Osteoarthritis, Knee / blood*,  etiology,  genetics
RNA, Untranslated / blood*
Reverse Transcriptase Polymerase Chain Reaction
Young Adult
Grant Support
5RC1AR58728-2/AR/NIAMS NIH HHS; ONT 68722//Canadian Institutes of Health Research; RC1 AR058728/AR/NIAMS NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/RNA, Untranslated

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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