Document Detail

Serum and mucosal antibody responses and protection in pigs vaccinated against Mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant.
MedLine Citation:
PMID:  9258425     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the protective efficacy of a pool of denatured membrane protein antigens of Mycoplasma hyopneumoniae (J strain) in the molecular size range 70 to 85 kDa (F3 antigen) in combination with adjuvants for pigs challenged with M hyopneumoniae. DESIGN: A vaccine efficacy experiment with assessment of serum and respiratory tract antibody responses. PROCEDURE: F3 antigens were emulsified with five different adjuvants. To groups of three pigs per vaccine, four vaccines were given by intramuscular injection, and two vaccines, including one of those given intramuscularly, were given by intraperitoneal injection. RESULTS: Compared to six unvaccinated pigs, animals vaccinated with F3 antigen displayed significantly reduced pneumonia (54% reduction in mean lung score) following experimental challenge. Analysis of post-vaccination, pre-challenge IgG and IgA ELISA antibody absorbances in serum and respiratory tract washings revealed no correlation with lung score. Six weeks after challenge, pigs previously vaccinated intramuscularly mostly demonstrated greater IgG and IgA responses in respiratory tract washings, and greater IgG serum antibody responses, than those vaccinated by intraperitoneal injection. CONCLUSION: Pigs vaccinated with M hyponeumoniae antigens in the molecular size range of 70 to 85 kDa showed a significant reduction in lung lesions compared with unvaccinated control animals after experimental challenge. IgG and IgA antibody concentrations in serum and respiratory tract washings after vaccination do not provide a useful prognostic indicator of protection from enzootic pneumonia.
S P Djordjevic; G J Eamens; L F Romalis; P J Nicholls; V Taylor; J Chin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Australian veterinary journal     Volume:  75     ISSN:  0005-0423     ISO Abbreviation:  Aust. Vet. J.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-12-09     Completed Date:  1997-12-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370616     Medline TA:  Aust Vet J     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  504-11     Citation Subset:  IM    
NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales.
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MeSH Terms
Adjuvants, Immunologic / administration & dosage
Antibodies, Bacterial / analysis,  biosynthesis*,  blood
Antigens, Bacterial / administration & dosage,  immunology
Bacterial Vaccines / administration & dosage,  immunology*
Enzyme-Linked Immunosorbent Assay / methods,  veterinary
Immunoglobulin A / analysis,  biosynthesis,  blood
Immunoglobulin G / analysis,  biosynthesis,  blood
Injections, Intramuscular / methods,  veterinary
Injections, Intraperitoneal / methods,  veterinary
Membrane Proteins / administration & dosage,  immunology
Molecular Weight
Mucous Membrane / chemistry,  immunology,  pathology
Mycoplasma / immunology*
Pneumonia of Swine, Mycoplasmal / immunology,  prevention & control,  veterinary*
Respiratory System / chemistry,  immunology,  pathology
Swine Diseases / epidemiology,  immunology,  prevention & control*
Vaccination / methods,  veterinary*
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Antibodies, Bacterial; 0/Antigens, Bacterial; 0/Bacterial Vaccines; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Membrane Proteins

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