Document Detail


Serum and mucosal S100 proteins, calprotectin (S100A8/S100A9) and S100A12, are elevated at diagnosis in children with inflammatory bowel disease.
MedLine Citation:
PMID:  17852869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Various markers characterize the complex inflammatory processes seen in chronic inflammatory bowel disease (IBD) including calprotectin, a complex of two S100 proteins, which has been evaluated and validated as a faecal marker of inflammation. However, the systemic and mucosal expression patterns of calprotectin and related S100 proteins are not well characterized in this disease. The objective of this study was to assess serum and mucosal levels of calprotectin, S100A12 and soluble receptor for advanced glycation end products (sRAGE), a putative S100 ligand, in a paediatric population with IBD. MATERIAL AND METHODS: Children were enrolled at diagnosis of IBD, along with groups of children without IBD. Standard inflammatory markers and disease activity scores were collated. Calprotectin, S100A12 and sRAGE levels in serum and biopsy culture supernatants were measured by ELISA and tissue distribution of S100 proteins was investigated by immunohistochemistry. RESULTS: Serum and mucosal calprotectin and S100A12 levels were increased in children with IBD as compared with non-IBD controls. Serum calprotectin levels correlated with S100A12 levels and with disease activity scores in children with IBD. sRAGE levels were not increased in IBD. S100A8, S100A9 and S100A12 were abundantly expressed throughout the lamina propria and epithelium in inflamed mucosa. In contrast, these proteins were present in the lamina propria, but not the epithelium, in non-inflamed mucosa. CONCLUSIONS: Serum calprotectin and S100A12 are increased in children with IBD and indicate disease activity. Elevated levels of these proteins are present in the colonic mucosa and may contribute to the pathogenesis of IBD. Furthermore, an imbalance between sRAGE and S100A12 may contribute to inflammatory changes present in IBD.
Authors:
Steven T Leach; Zheng Yang; Isabella Messina; Changjie Song; Carolyn L Geczy; Anne M Cunningham; Andrew S Day
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  42     ISSN:  0036-5521     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1321-31     Citation Subset:  IM    
Affiliation:
School of Women's and Children's Health, School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Biopsy
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Epithelium / chemistry
Female
Humans
Inflammatory Bowel Diseases / pathology*
Intestinal Mucosa / chemistry
Leukocyte L1 Antigen Complex / analysis*,  blood
Male
Mucous Membrane / chemistry
Receptors, Immunologic / blood
S100 Proteins / analysis*,  blood
Chemical
Reg. No./Substance:
0/Leukocyte L1 Antigen Complex; 0/Receptors, Immunologic; 0/S100 Proteins; 0/S100A12 protein, human; 0/advanced glycosylation end-product receptor

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