Document Detail


Serum and monohydroxytamoxifen inhibit progesterone receptor concentrations in primary rat uterine cells grown in serum-free medium.
MedLine Citation:
PMID:  2721449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Uterine cells dispersed from immature rat uteri were grown in culture in serum-free medium supplemented with insulin (5 micrograms/ml), transferrin (5 micrograms/ml), and selenous acid (5 ng/ml; ITS). Cells were grown to a confluent monolayer and had a fibroblastic appearance, similar to cells cultured in the presence of 10% serum [horse-fetal calf (6:1), stripped three times with dextran-coated charcoal]. However, estrogen receptor and progesterone receptor (PgR) concentrations were higher in cells grown in serum-free medium than in cells grown in serum. Uterine cells grown in ITS contain PgR levels that are near maximum (927 +/- 168 fmol/mg protein), and estradiol (0.1 nM; 24 h) causes a 10-50% increase in PgR. Addition of serum or monohydroxytamoxifen for 24 h decreases PgR levels by about 50%, and estrogen can completely overcome the inhibition caused by either serum or monohydroxytamoxifen. Our data suggest that PgR levels may be down-regulated by a component of serum.
Authors:
T L Greco; J Gorski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  124     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-06-28     Completed Date:  1989-06-28     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2837-43     Citation Subset:  AIM; IM    
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood
Cells, Cultured
Culture Media
Female
Kinetics
Rats
Rats, Inbred Strains
Receptors, Estrogen / drug effects,  metabolism
Receptors, Progesterone / biosynthesis,  drug effects,  metabolism*
Tamoxifen / analogs & derivatives*,  pharmacology*
Uterus / cytology,  drug effects,  metabolism*
Grant Support
ID/Acronym/Agency:
CA-18110/CA/NCI NIH HHS; HD-07259/HD/NICHD NIH HHS; HD-08192/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Receptors, Estrogen; 0/Receptors, Progesterone; 10540-29-1/Tamoxifen; 17197F0KYM/afimoxifene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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