Document Detail


Serum microRNA expression profile as a biomarker for the diagnosis of pertussis.
MedLine Citation:
PMID:  23073777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pertussis is a highly contagious, respiratory disease associated with substantial morbidity and mortality. A rapid and reliable diagnostic method is essential for appropriate treatment and prevention. Expression profiles of circulating microRNAs (miRNAs) have been proven as new non-invasive biomarkers for infectious diseases. We aimed to investigated the serum miRNA profile in pertussis patients and explored its potential as a novel diagnostic biomarker for pertussis. Among 664 different miRNAs analyzed using a miRNA array, 50 were overexpressed and 81 were underexpressed in the serum of pertussis patients. Expression levels of seven candidate miRNAs were further evaluated by real-time qRT-PCR. A panel of five miRNAs (miR-202, miR-342-5p, miR-206, miR-487b, miR-576-5p) was confirmed overexpressed in pertussis patients (p < 0.05). Risk score and receiver-operating characteristic (ROC) analysis showed that the area under the curve of the five-member miRNA profile was 0.980. At an optimal cutoff value (0.707), this panel of miRNAs yielded a sensitivity of 97.4 % and a specificity of 94.3 %. These data suggest that the five-member serum miRNA profile may serve as a new biomarker for pertussis diagnosis with high specificity and sensitivity.
Authors:
Yiyue Ge; Kangchen Zhao; Yuhua Qi; Xiaoyan Min; Zhiyang Shi; Xian Qi; Yunfeng Shan; Lan Cui; Minghao Zhou; Yong Wang; Hua Wang; Lunbiao Cui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-18
Journal Detail:
Title:  Molecular biology reports     Volume:  40     ISSN:  1573-4978     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-07     Completed Date:  2013-06-17     Revised Date:  2014-08-12    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1325-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Area Under Curve
Biological Markers / blood
Case-Control Studies
Female
Gene Expression Regulation
Humans
Infant
Male
MicroRNAs / blood*
ROC Curve
Real-Time Polymerase Chain Reaction
Risk
Transcriptome
Whooping Cough / blood*,  diagnosis*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/MIRN576 microRNA, human; 0/MicroRNAs

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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