Document Detail


Serum microRNA characterization identifies miR-885-5p as a potential marker for detecting liver pathologies.
MedLine Citation:
PMID:  20815808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Circulating miRNAs (microRNAs) are emerging as promising biomarkers for several pathological conditions, and the aim of this study was to investigate the feasibility of using serum miRNAs as biomarkers for liver pathologies. Real-time qPCR (quantitative PCR)-based TaqMan MicroRNA arrays were first employed to profile miRNAs in serum pools from patients with HCC (hepatocellular carcinoma) or LC (liver cirrhosis) and from healthy controls. Five miRNAs (i.e. miR-885-5p, miR-574-3p, miR-224, miR-215 and miR-146a) that were up-regulated in the HCC and LC serum pools were selected and further quantified using real-time qPCR in patients with HCC, LC, CHB (chronic hepatitis B) or GC (gastric cancer) and in normal controls. The present study revealed that more than 110 miRNA species in the serum samples and wide distribution ranges of serum miRNAs were observed. The levels of miR-885-5p were significantly higher in sera from patients with HCC, LC and CHB than in healthy controls or GC patients. miR-885-5p yielded an AUC [the area under the ROC (receiver operating characteristic) curve] of 0.904 [95% CI (confidence interval), 0.837-0.951, P<0.0001) with 90.53% sensitivity and 79.17% specificity in discriminating liver pathologies from healthy controls, using a cut off value of 1.06 (normalized). No correlations between increased miR-885-5p and liver function parameters [AFP (α-fetoprotein), ALT (alanine aminotransferase), AST (aspartate aminotransferase) and GGT (γ-glutamyl transpeptidase)] were observed in patients with liver pathologies. In summary, miR-885-5p is significantly elevated in the sera of patients with liver pathologies, and our data suggest that serum miRNAs could serve as novel complementary biomarkers for the detection and assessment of liver pathologies.
Authors:
Junhao Gui; Yaping Tian; Xinyu Wen; Wenhui Zhang; Pengjun Zhang; Jing Gao; Wei Run; Liyuan Tian; Xingwang Jia; Yanhong Gao
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  120     ISSN:  1470-8736     ISO Abbreviation:  Clin. Sci.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2010-11-22     Completed Date:  2011-02-03     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-93     Citation Subset:  IM    
Affiliation:
Department of Clinical Biochemistry, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing 100853, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biological Markers / blood
Carcinoma, Hepatocellular / diagnosis
Epidemiologic Methods
Female
Gene Expression Profiling / methods
Humans
Liver Cirrhosis / diagnosis
Liver Diseases / diagnosis*
Liver Neoplasms / diagnosis
Male
MicroRNAs / blood*
Middle Aged
Polymerase Chain Reaction / methods
RNA, Neoplasm / blood
Tumor Markers, Biological / blood
Up-Regulation
Chemical
Reg. No./Substance:
0/Biological Markers; 0/MicroRNAs; 0/RNA, Neoplasm; 0/Tumor Markers, Biological
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