| Serum matrix metalloproteinase-2 levels indicate blood-CSF barrier damage in patients with infectious meningitis. | |
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MedLine Citation:
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PMID: 18185989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Protein components in cerebrospinal fluid (CSF) are maintained at a specific concentration by a dynamic gradient between the capillary and intrathecal spaces via the blood-cerebrospinal fluid barrier (BCB) in the brain and spinal cord. Permeability to proteins increases when there is structural damage to the BCB. Matrix metalloproteinase-2 (MMP-2; gelatinase A) has been shown to degrade type IV collagen, a major component of the cellular basement membrane. We analyzed alpha2 macroglobulin (alpha2M) indices and evaluated the relationship between alpha2M, as an indicator of BCB permeability, and MMP-2, which degrades the extra-cellular matrix in patients with infectious meningitis. MATERIALS AND METHODS: Albumin levels in CSF or serum were determined by turbidimetric immunoassay, or bromcresol green assay, respectively. alpha2M levels in CSF or serum were measured with enzyme-linked immunosorbent assay, or laser-nephelometry, respectively. Serum MMP-2 levels were determined by enzyme immuno assay. We calculated the alpha2M index, i.e. the ratio of alpha2M (CSF / serum) to albumin (CSF / serum; alpha2M in CSF / alpha2M in serum x albumin in serum / albumin in CSF). RESULTS: alpha2M indices were significantly increased in infectious meningitis compared to healthy controls (p < 0.05). They were highest in bacterial meningitis, and there was a significant difference between viral or mycotic and bacterial meningitis (p < 0.05). Serum MMP-2 levels were increased in infectious meningitis, being highest in bacterial meningitis, where they were significantly different from healthy controls (p < 0.05). There was a significant positive correlation between serum MMP-2 levels and alpha2M indices (r = 0.64, p < 0.0001). CONCLUSION: Markedly increased levels of serum MMP-2 in infectious, especially bacterial, meningitis may reflect the degree of damage to the BCB. |
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Authors:
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Yuhsaku Kanoh; Tadashi Ohara; Motonari Kanoh; Tohru Akahoshi |
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Publication Detail:
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Type: Journal Article Date: 2008-01-10 |
Journal Detail:
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Title: Inflammation Volume: 31 ISSN: 0360-3997 ISO Abbreviation: Inflammation Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-13 Completed Date: 2008-05-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: United States |
Other Details:
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Languages: eng Pagination: 99-104 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan. kanoh@med.kitasato-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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blood,
cerebrospinal fluid Blood-Brain Barrier / metabolism*, pathology Capillary Permeability Case-Control Studies Enzyme-Linked Immunosorbent Assay Humans Matrix Metalloproteinase 2 / blood* Meningitis, Bacterial / cerebrospinal fluid, enzymology*, pathology Meningitis, Fungal / cerebrospinal fluid, enzymology*, pathology Meningitis, Viral / cerebrospinal fluid, enzymology*, pathology Nephelometry and Turbidimetry Serum Albumin / cerebrospinal fluid Up-Regulation alpha-Macroglobulins / cerebrospinal fluid* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Serum Albumin; 0/alpha-Macroglobulins; EC 3.4.24.24/MMP2 protein, human; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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