Document Detail

Serum-induced G0/G1 transition in chemically transformed 3T3 cells. Independence of protein synthesis, stable "memory", and enhancement by cycloheximide pretreatment.
MedLine Citation:
PMID:  3545864     Owner:  NLM     Status:  MEDLINE    
Quiescent, chemically transformed (benzo-alpha-pyrene) BALB/c 3T3 cells (BP A31) enter the cell division cycle when exposed to complete medium containing 10% fetal calf serum (FCS); the number of cells recruited is a function of the duration of serum exposure. The recruitment of cells by short (less than 4 h) serum pulses is not inhibited by simultaneous exposure to cycloheximide (CH), and therefore the initial commitment does not require protein synthesis. The cells enter S phase with a constant delay following the removal of CH, even if CH exposure has been continued for as long as 20 h after the end of the serum pulse. The cell recruitment by serum pulses was inhibited by 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (DRB), an inhibitor of cytoplasmic mRNA accumulation. These data suggest that serum exposure produces a stable 'memory' that is necessary and sufficient for the eventual progression through G1 to S phase that occurs when protein synthesis is resumed after the removal of CH; this 'memory' probably consists of mRNA species that are induced by serum and that are stable in the absence of protein synthesis. Unexpectedly, pretreatment of quiescent BP A31 cells with CH (8-24 h) dramatically increased the fraction of the total cell population that is recruited by a serum pulse of fixed duration.
H E Gray; T Buchou; J Mester
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  169     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-04-08     Completed Date:  1987-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  95-104     Citation Subset:  IM    
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MeSH Terms
Benzo(a)pyrene / pharmacology*
Cell Transformation, Neoplastic*
Cells, Cultured
Culture Media
Insulin / pharmacology
Interphase* / drug effects
Mice, Inbred BALB C
Reg. No./Substance:
0/Culture Media; 11061-68-0/Insulin; 50-32-8/Benzo(a)pyrene

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