Document Detail

Serum from radiofrequency-injured livers induces differentiation of bone marrow stem cells into hepatocyte-like cells.
MedLine Citation:
PMID:  19394655     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Roles that bone marrow stem cells (BMSCs) play in liver repair after liver injury and the cell therapy for liver diseases are widely accepted. However, the availability of hepatocyte-like cells from BMSCs and possible animal diseases association with culturing in fetal calf serum (FCS) are the major limitations of clinical therapy. Therefore, this study was designed to search for a new cell source for the treatment of liver injuries through investigating whether serum from radiofrequency ablation-injured rabbit livers can induce the differentiation of BMSCs into hepatocyte-like cells. METHODS: Serum was collected from rabbits 36 h after radiofrequency ablation (RFA) treatment of the liver. BMSCs were isolated from rabbit bone marrow and were cultured in the collected serum. Cellular morphology and cell cycle were observed. Hepatocyte markers of the differentiated cells were detected by immunohistochemistry. RESULTS: After induction for 7 d, spindle-shaped BMSCs turned into round cells that resembled the morphology of hepatocyte-like cells. Flow cytometry showed that the percentage of cells in the S/G2/M phase was higher in the RFA group than that in the FCS group and HGF groups. This result suggests that BMSC can transform into mature cells from stem cell phase. Albumin and CK18 were considered as typical marker of hepatocytes. Following induction for 14 d, the differentiated cells expressed immunofluorescence of FITC-labeled albumin and TRITC-labeled CK18. CONCLUSION: BMSCs treated with serum collected from radiofrequency ablation-injured livers can differentiate into hepatocyte-like cells providing a cell source to cell therapy.
Yi Yang; Bo Qu; Jian-Hua Huo; Sheng-Li Wu; Ming-Yu Zhang; Zuo-Ren Wang
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Publication Detail:
Type:  Journal Article     Date:  2009-01-24
Journal Detail:
Title:  The Journal of surgical research     Volume:  155     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-07-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  IM    
Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China.
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MeSH Terms
Blood Transfusion, Autologous*
Bone Marrow Cells / cytology*
Catheter Ablation*
Cell Cycle
Cell Differentiation*
Flow Cytometry
Fluorescent Antibody Technique
Hepatocytes / cytology*
Liver Neoplasms / therapy
Liver Regeneration*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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