| Serum-free medium for murine and human lymphoid and hybridoma cell lines. | |
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MedLine Citation:
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PMID: 1367398 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We established a serum-free medium of low protein content (125 micrograms/ml) TYI 100, consisting of three hormones and five growth factors for the growth of lymphoid and hybridoma cell lines. In TYI 100 medium, mouse and human hybridomas grew equally well as in RPMI 1640 supplemented with 10% fetal bovine serum (10% FBS) without adaptation to the serum-free medium. TYI 100 medium allowed several passages of mouse hybridoma lines and the total cell number was more than in 10% FBS. TYI 100 medium also supported growth of myelomas and anchorage dependent cell lines, Bowes and CHO, well. TYI 100 medium is composed of inexpensive supplements and is therefor applicable to large scale culture. |
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Authors:
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M Togami; K Yasuda; M Kariya |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 6 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-10-17 Completed Date: 1991-10-17 Revised Date: 2008-10-09 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 33-8 Citation Subset: B |
Affiliation:
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Tsukuba Research Laboratory, Japan Synthetic Rubber Co., Ltd. Ibaraki. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Cell Division Cells, Cultured Culture Media* Growth Substances / pharmacology Hormones / pharmacology Humans Hybridomas / cytology* Kinetics Lymphocytes / cytology* Mice |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Growth Substances; 0/Hormones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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