Document Detail

Serum-free cell culture: the serum-free media interactive online database.
MedLine Citation:
PMID:  20390239     Owner:  NLM     Status:  MEDLINE    
Fetal bovine serum (FBS) is a ubiquitously used essential supplement in cell culture media. However, there are serious scientific and ethical concerns about the use of FBS regarding its harvest and production. During the last three decades, FBS could be substituted by other supplements or by the use of defined chemical components in serum-free cell culture. A number of serum-free medium formulations have been described for mammalian and insect cell lines as well as for primary cultures. However, the switch to serum-free media still demands a time-consuming literature survey and a manufacturer search for appropriate medium formulations, respectively. Here we present the second collection of commercially available serum-free media in an updated, freely accessible interactive online database. Searches for serum-free media and continuous cell lines already adapted to serum-free culture can be performed according to various criteria. These include the degree of chemical definition, e.g. serum-free (SF), animal-derived component free (ADCF) or chemically defined (CD), and the type of medium, e.g. basal media, medium supplements, or full replacement media. In order to specify the cell lines that are adapted to serum-free media, search terms like species, organ, tissue, cell type and disease can be used. All commercially available serum-free media and adapted cell lines currently available from major distributors (e.g. ATCC, ECACC and DMSZ) are included in the database. Despite an extensive search for serum-free media and adapted cell lines, detailed information from certain companies and suppliers is still lacking and is specifically highlighted. It is intended to create a platform for the interactive exchange of information and experience by experts in the field in order to continuously improve and extend the serum-free online database. The database is accessible at
Daniel Brunner; Jürgen Frank; Helmut Appl; Harald Schöffl; Walter Pfaller; Gerhard Gstraunthaler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  ALTEX     Volume:  27     ISSN:  1868-596X     ISO Abbreviation:  ALTEX     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-14     Completed Date:  2010-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100953980     Medline TA:  ALTEX     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  53-62     Citation Subset:  IM    
zet - Centre for Alternative and Complementary Methods to Animal Testing, Linz, Austria.
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MeSH Terms
Animal Testing Alternatives
Animal Welfare
Cell Culture Techniques / ethics,  methods*
Culture Media, Serum-Free*
Databases, Factual*
Reg. No./Substance:
0/Culture Media, Serum-Free

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