Document Detail

Serum fibrinogen alpha C-chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus.
MedLine Citation:
PMID:  23382097     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Clinical application of biomarker candidates discovered by proteomic analysis is challenging. The purpose of this study was to standardize preanalytical conditions for measurement of serum levels of fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9) and to test the diagnostic value of this peptide for detection of early hepatic fibrosis in patients with hepatitis C virus (HCV)-related chronic hepatitis.
EXPERIMENTAL DESIGN: Serum FIC 5.9 levels were measured by a sandwich ELISA. Effects on the serum FIC 5.9 level of temperature, the time between venipuncture and serum separation, and the types of collection tubes used were examined. The diagnostic value of serum FIC 5.9 as an early indicator of hepatic fibrosis due to HCV was then assessed.
RESULTS: FIC 5.9 was produced in a time- and temperature-dependent manner after venipuncture. Abnormal FIC 5.9 values were found in 89.5% of FI stage patients. Receiver operating characteristic analyses confirmed the superiority of FIC 5.9 over other conventional markers for early detection of fibrosis.
CONCLUSIONS AND CLINICAL RELEVANCE: The serum FIC 5.9 level may be an early indicator of hepatic fibrosis in HCV-related chronic liver diseases. This study provides an example of a pipeline from biomarker discovery by proteome analysis to assay optimization and preliminary clinical validation.
Kazuyuki Sogawa; Kenta Noda; Hiroshi Umemura; Masanori Seimiya; Takahisa Kuga; Takeshi Tomonaga; Motoi Nishimura; Fumihiko Kanai; Fumio Imazeki; Hirotaka Takizawa; Masato Yoneda; Atsushi Nakajima; Mikihiro Tsutsumi; Osamu Yokosuka; Fumio Nomura
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Publication Detail:
Type:  Journal Article     Date:  2013-05-17
Journal Detail:
Title:  Proteomics. Clinical applications     Volume:  7     ISSN:  1862-8354     ISO Abbreviation:  Proteomics Clin Appl     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-19     Completed Date:  2013-11-27     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  101298608     Medline TA:  Proteomics Clin Appl     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  424-31     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Biological Markers / blood
Blood Specimen Collection / instrumentation,  methods
Early Diagnosis
Enzyme-Linked Immunosorbent Assay / standards
Fibrinogen / standards
Hepacivirus / pathogenicity*
Hepatitis C, Chronic / complications,  diagnosis
Liver Cirrhosis / blood,  diagnosis*,  etiology
Middle Aged
Peptide Fragments / blood*,  standards
ROC Curve
Reference Values
Time Factors
Reg. No./Substance:
0/Biological Markers; 0/Peptide Fragments; 0/fibrinogen alphaC; 9001-32-5/Fibrinogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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