Document Detail


Serum factors that stimulate fatty acid oxidation: physiological specificity.
MedLine Citation:
PMID:  3944194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the accompanying paper (Wice et al., 1986) we reported that serum from chickens contains small molecular weight compounds that stimulate long-chain fatty acid oxidation ten fold or more in HeLa cells. Here we show that this response is not limited to specific sera or to specific target cells. The specificity of the metabolic response to these factors was also investigated. They had no effect on the following major pathways of HeLa cell metabolism: 1) the oxidation of the medium-chain fatty acid, octanoic acid, 2) the rate of glycolysis of glucose, 3) the flux of glucose carbon through the oxidative arm of the pentose cycle, 4) the entry of pyruvate into the citrate cycle, 5) the oxidation of glutamine carbon, 6) the utilization rate of oxygen or 7) the rate of fatty acid synthesis. Furthermore, the increased oxidation of long-chain fatty acids was not a result of an increased uptake into the cells. Thus, the serum factors appear to be very specific for the oxidation of long-chain fatty acids for energy. Since carnitine also stimulates long-chain fatty acid oxidation in these cells, it seems likely that these compounds either facilitate the activity of carnitine or provide the same function--presumably the transport of long-chain fatty acid into and out of the mitochondria.
Authors:
J Stanisz; B M Wice; D E Kennell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  126     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-03-06     Completed Date:  1986-03-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  141-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Physiological Phenomena*
Carnitine / physiology
Cells, Cultured
Chick Embryo
Fatty Acids / metabolism*
Fibroblasts / metabolism
Glucose / metabolism
Glutamine / metabolism
Hela Cells
Humans
Mitochondria / metabolism
Oxidation-Reduction
Oxygen Consumption
Chemical
Reg. No./Substance:
0/Fatty Acids; 50-99-7/Glucose; 541-15-1/Carnitine; 56-85-9/Glutamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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