| Serum erythropoietin and hemoglobin affinity for oxygen in patients phlebotomized for polycythemia vera. | |
| | |
MedLine Citation:
|
PMID: 2486292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Serum erythropoietin (Ep) was measured by radioimmunoassay before and 24 hours after therapeutic phlebotomies in patients with polycythemia vera (PV) and in normal subjects before and after phlebotomies of comparable volumes. In addition the in vivo oxygen affinity of hemoglobin (P50) was calculated, and red cell indices and 2,3 DPG values were measured. Paired t tests determined whether the differences between pre- and post--phlebotomy values were statistically significant. Blood hemoglobin (Hb) levels declined after phlebotomy, and generally continued to be at or above normal levels. Serum Ep increased after phlebotomy in both groups of subjects. The in vivo P50 value for patients with PV (29.4 +/- 0.4 mmHg) was significantly (p less than 0.005) greater than the normal value (27.2 +/- 0.5). Seven of the PV patients (5 males, 2 females) were restudied. Their Hb and hematocrit values were either normal or slightly higher than normal. The MCV for 4 males and 1 female was below normal. The MCHC was slightly lower than normal and reticulocytosis was not present. The male PV patients had greater than normal 2,3 DPG values and most had right-shifted P50 values. There was no correlation between 2,3 DPG values and P50 values. The female patients did not have 2,3 DPG values consistently greater than normal and their P50 values were not right shifted. These results showed that serum EP increased in response to small reductions in Hb even when subnormal Hb values were not produced. The reduced affinity of Hb in PV patients may explain earlier observations that patients with PV have lower levels of urinary or plasma Ep than normals with the same hematocrits. |
| | |
Authors:
|
R A Cohen; G Clemons; S Ebbe |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Advances in experimental medicine and biology Volume: 271 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 1989 |
Date Detail:
|
Created Date: 1991-01-23 Completed Date: 1991-01-23 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 9-15 Citation Subset: IM |
Affiliation:
|
Lawrence Berkeley Laboratory, University of California, Berkeley 94720. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
2,3-Diphosphoglycerate Adult Bloodletting* Diphosphoglyceric Acids / blood Erythrocyte Indices Erythropoietin / blood*, secretion Female Hemoglobins / metabolism* Humans Male Oxygen / metabolism* Polycythemia Vera / blood*, therapy |
| Chemical | |
Reg. No./Substance:
|
0/Diphosphoglyceric Acids; 0/Hemoglobins; 11096-26-7/Erythropoietin; 138-81-8/2,3-Diphosphoglycerate; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cellular localization of erythropoietin gene transcription.
Next Document: Effects of hemin on erythropoiesis.