| Serum creatinine determined by jaffe, enzymatic method, and isotope dilution-liquid chromatography-mass spectrometry in patients under hemodialysis. | |
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MedLine Citation:
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PMID: 22628238 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVES: Serum creatinine is an important clinical marker for renal clearance. However, the Jaffe method had much interference and the accuracy had not been tested in patients under hemodialysis (HD) with standard isotope dilution-liquid chromatography-mass spectrometry (IDLCMS) method. The validity of enzymatic method is also unknown. METHODS: The predialysis serum creatinine levels of 126 patients under regular HD for 3 months were checked by Jaffe, enzymatic, and IDLCMS methods. We compared the value of the Jaffe and enzymatic to that of IDLCMS in linear regression model. And we also tried to find the clinical parameters that influence the difference between Jaffe vs. IDLCMS and enzymatic vs. IDLCMS method. RESULTS: We found significant underestimate serum creatinine in uremic patients by Jaffe and enzymatic methods. Serum glucose and globulin are positive biases, whereas albumin, potassium, and phosphorus are negative biases. Enzymatic method is less affected by serum glucose and serum protein. Albumin acts differently in uremic serum compared to the results of mixing them with normal serum. CONCLUSIONS: For uremic patients, in whom creatinine level is high and many of them suffered from diabetes mellitus, serum creatinine can be either under- or overestimated by Jaffe method. Enzymatic method is less affected and may be a better method. J. Clin. Lab. Anal. 26:206-214, 2012. © 2012 Wiley Periodicals, Inc. |
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Authors:
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Wen-Sheng Liu; Yu-Ting Chung; Chih-Yu Yang; Chih-Ching Lin; Kun-Hung Tsai; Wu-Chang Yang; Tzen-Wen Chen; Yen-Ting Lai; Szu-Yuan Li; Tsung-Yun Liu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical laboratory analysis Volume: 26 ISSN: 1098-2825 ISO Abbreviation: J. Clin. Lab. Anal. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8801384 Medline TA: J Clin Lab Anal Country: United States |
Other Details:
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Languages: eng Pagination: 206-14 Citation Subset: IM |
Copyright Information:
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© 2012 Wiley Periodicals, Inc. |
Affiliation:
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Division of Nephrology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.; School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.; Division of Nephrology, Department of Medicine, Taipei City Hospital, Zhongxing Branch, Taiwan, R.O.C. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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