| Serum carboxypeptidase A activity as a biomarker for early-stage pancreatic carcinoma. | |
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MedLine Citation:
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PMID: 17222396 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The early stage of pancreatic carcinoma lacks typical clinical signs and symptoms, and is difficult to diagnose. We developed an assay for active form of serum carboxypeptidase A (F-CPA) and its zymogen precursor pro CPA, and evaluated them as a marker for early-stage pancreatic carcinoma. METHODS: Serum CPA from 406 patients including 169 with pancreatic carcinoma, 53 with acute pancreatitis, 23 with chronic pancreatitis, and 161 with non-pancreatic diseases were assayed by a method with N-acetyl-phenylalanyl-l-3-thiaphenylalanine as substrate and dl-benzylsuccinic acid as specific inhibitor of CPA. Activation of pro CPA was carried out using trypsin. RESULTS: An established assay system was performed fully automatically and possesses enough performance for routine clinical assay. This system requires 31 minutes for CPA detection. No significant differences were detected between the F-CPA activity of patients with pancreatic carcinoma and that of patients with non-pancreatic diseases (p=0.168). F-CPA was mainly increased in patients with pancreatitis. Since total-CPA (T-CPA, T-CPA=pro CPA+F-CPA) was increased both in patients with pancreatic carcinoma and those with acute pancreatitis (p<0.0001, each case), the F-CPA/T-CPA ratio was low only in the patients with pancreatic carcinoma. The rate of positivity of T-CPA in the early stage of pancreatic carcinoma in which tumor was less than 2 cm was 77%, and higher than those of CA19-9 (31%), CEA (8%), and elastase 1 (46%). CONCLUSION: It was suggested that assays of both T-CPA and F-CPA in serum might be useful for the surveillance of early-stage pancreatic carcinoma. |
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Authors:
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Shinji Matsugi; Takatoshi Hamada; Noriko Shioi; Takumi Tanaka; Takashi Kumada; Shinji Satomura |
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Publication Detail:
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Type: Journal Article Date: 2006-11-24 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 378 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-19 Completed Date: 2007-04-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 147-53 Citation Subset: IM |
Affiliation:
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New Diagnostics Business and Technology Development Department, Wako Pure Chemical Industries, Ltd., 6-1 Takada-cho, Amagasaki, Hyogo, Japan. matsugi.shinji@wako-chem.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carboxypeptidases A
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blood* Enzyme Activation Enzyme Precursors / blood Humans Neoplasm Staging Pancreatic Neoplasms / enzymology* ROC Curve Tumor Markers, Biological / blood* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Precursors; 0/Tumor Markers, Biological; EC 3.4.17.1/Carboxypeptidases A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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