Document Detail


Serum can act as a shear protecting agent in agitated hybridoma cell cultures.
MedLine Citation:
PMID:  2693339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hybridoma cells, S3H5/gamma 2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.
Authors:
G M Lee; J M Savinell; B O Palsson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Hybridoma     Volume:  8     ISSN:  0272-457X     ISO Abbreviation:  Hybridoma     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-03-07     Completed Date:  1990-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8202424     Medline TA:  Hybridoma     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  639-45     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, University of Michigan, Ann Arbor.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Culture Media*
Cytological Techniques
Hybridomas / cytology*
Stress, Mechanical
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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