Document Detail


Serum antibodies against the heat shock protein 60 are elevated in patients with osteosarcoma.
MedLine Citation:
PMID:  10776793     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Osteosarcoma is the most frequent malignant bone tumor, mainly occurring in the second and third decade of life. Diagnosis is limited to clinical symptoms, radiology and histology, but so far no diagnostic laboratory tests are available. Heat shock proteins (hsp), highly conserved proteins performing vital intracellular chaperoning functions and preventing cells from death, have been shown to be involved in tumor immunity. We analyzed 75 sera from 23 patients with high-grade osteosarcoma, 8 patients with chondrosarcoma, 10 patients with Ewing's sarcoma, 5 patients with soft tissue sarcoma, 11 patients with benign bone tumors at the time of diagnosis and from 18 healthy controls with an indirect one-site enzyme linked immunosorbent assay (ELISA) for the presence of anti-hsp60 and 70 antibodies. In these assays 10/23 osteosarcoma patients (43%) had anti-hsp60 antibodies with a mean +/- S.D. titer of 0.382 +/- 0.243 U/ml. Only one of the 18 healthy controls (1/18, 5.6%; titer 0.22 U/ml), two of the Ewing's sarcoma patients (2/10, 20%; titer 0.2 +/- 0.09 U/ml), two of the patients with a benign bone tumor (2/11, 18%; titer 0.22 +/- 0.16 U/ml) and one of the chondrosarcoma patients (1/8, 12.5%; titer 0.14 U/ml) were positive, whereas all others, including all soft tissue sarcomas were negative throughout. Anti-hsp60 antibodies in patients with osteosarcoma are therefore significantly increased (p < 0.05). 19/23 (83%) of osteosarcoma biopsy specimens expressed hsp60 immunohistochemically and all specimens from patients with a positive anti-hsp60 serum titer expressed hsp60. The level of the anti-hsp60 antibodies did not correlate with clinical parameters such as response to preoperative chemotherapy, duration of symptoms, age, gender, tumor size, serum alkaline-phosphatase levels and metastases. Although no difference in anti-hsp70 antibodies could be observed between sera from patients and healthy controls, a positive correlation was found for the presence of anti-hsp70 serum antibodies and lung metastases at the time of diagnosis in osteosarcoma patients. These data suggest an increase of anti-hsp60 antibodies at the time of first diagnosis of osteosarcoma. These findings should therefore give rise to further investigations on a group of new markers for the diagnosis of osteosarcoma.
Authors:
K Trieb; R Gerth; R Windhager; J G Grohs; G Holzer; P Berger; R Kotz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunobiology     Volume:  201     ISSN:  0171-2985     ISO Abbreviation:  Immunobiology     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-07-27     Completed Date:  2000-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8002742     Medline TA:  Immunobiology     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  368-76     Citation Subset:  IM    
Affiliation:
Department of Orthopedics, University of Vienna, Austria. Klemens.Trieb@akh-wien.ac.at
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Neoplasm / blood*,  immunology
Bone Neoplasms / blood,  immunology*,  pathology
Chaperonin 60 / immunology*
Child
Chondrosarcoma / blood,  immunology
Female
HSP70 Heat-Shock Proteins / immunology
Humans
Male
Middle Aged
Osteosarcoma / blood,  immunology*,  pathology
Sarcoma / blood,  immunology
Sarcoma, Ewing's / blood,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Neoplasm; 0/Chaperonin 60; 0/HSP70 Heat-Shock Proteins

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