Document Detail


Serum acid ribonuclease in myelogenous leukemia.
MedLine Citation:
PMID:  26465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acid and alkaline RNase activities in serum were measured with yeast RNA as the substrate in normal subjects and in leukemic patients pretreatment and posttreatment, and the acid/alkaline ratios of activities were 0.63 +/- 0.08 (S.D.) (N, 12), 2.28 +/- 0.82 (N, 8), and 0.60 +/- 0.13 (N, 9), respectively. The mean value for the ratio in the pretreated leukemia was significantly higher than that in the other 2 groups (p less than 0.01). By separating these acid and alkaline RNases from normal and leukemic sera by phosphocellulose chromatography, it was further confirmed that acid RNase alone increased markedly in leukemic serum. From serum and leukocytes of leukemic patients, acid RNases were purified about 2000-fold and 300-fold, respectively, by phosphocellulose and Sephadex G-75 chromatography. Both enzymes displayed properties nearly identical with those of normal serum and leukocytes, except that leukemic serum acid RNase had about a 2.4-fold greater affinity for polyuridylate than for polycytidylate as substrate, in contrast to normal serum acid RNase that degraded polycytidylate exclusively. On the other hand acid RNases from serum leukocytes of leukemia showed a similar substrate preference. These results suggest that the high RNase levels of leukemic sera are due to an excessive leakage of acid RNase into the blood stream from abnormal leukocytes.
Authors:
K Akagi; M Yamanaka; K Murai; Y Niho; T Omae
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  38     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1978 Jul 
Date Detail:
Created Date:  1978-08-14     Completed Date:  1978-08-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2168-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Chromatography
Female
Humans
Hydrogen-Ion Concentration
Leukemia, Myeloid / enzymology*
Leukemia, Myeloid, Acute / enzymology*
Leukocytes / enzymology
Male
Middle Aged
Poly C / metabolism
Poly U / metabolism
Ribonucleases / blood*,  isolation & purification
Substrate Specificity
Chemical
Reg. No./Substance:
27416-86-0/Poly U; 30811-80-4/Poly C; EC 3.1.-/Ribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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