Document Detail


Serum TGF-beta2 and TGF-beta3 are increased and positively correlated to pain, functionality, and radiographic staging in osteoarthritis.
MedLine Citation:
PMID:  20704110     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The goal of this study was to verify or reject the hypothesis that systematic differences exist in various profibrotic or antifibrotic factors between osteoarthritic patients and controls, as well as between different stages of osteoarthritis. The study group comprised 63 patients with knee osteoarthritis and 18 controls. Transforming growth factor-beta (TGF-beta)1, -2, -3; tissue inhibitor of metalloproteinase (TIMP)-1 protein levels; and gelatinolytic activity of matrix metalloproteinase (MMP)-1, -2, -3, -9 activities were measured by enzyme-linked immunosorbent assay and gelatin zymography, respectively. Visual analog scale scores, Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores, Lequesne clinical osteoarthritis scales, and Kellgren-Lawrence radiographic grading were recorded for each patient.Transforming growth factor-beta2 and -3 (in contrast to TGF-beta1 and TIMP-1) serum protein levels were significantly higher in osteoarthritic patients compared to controls (210%+/-14% [P<.001] and 232%+/-7% [P<10(-7)], respectively). Additionally, TGF-beta2 and -3 were strongly positively correlated to Kellgren-Lawrence radiographic grading of the disease (P<10(-5) and P<10(-7), respectively). Moreover, TGF-beta2 correlated positively with the WOMAC scale (P=.007). However, TIMP-1 decreased as osteoarthritis progressed clinically, but remained irrelevant to radiographic staging. Furthermore, activities of MMP-2 and -9, but not MMP-1+/-3, were lower in patients with osteoarthritis.
Authors:
Stilianos Kapetanakis; Ioannis Drygiannakis; Kostantinos Kazakos; Nikolaos Papanas; George Kolios; Elias Kouroumalis; Dionysios-Alexandros Verettas
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2010-08-11
Journal Detail:
Title:  Orthopedics     Volume:  33     ISSN:  1938-2367     ISO Abbreviation:  Orthopedics     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-13     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806107     Medline TA:  Orthopedics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  -     Citation Subset:  IM    
Copyright Information:
Copyright 2010, SLACK Incorporated.
Affiliation:
Department of Orthopedics, University Hospital of Alexandroupolis, Heraklion, Greece.
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MeSH Terms
Descriptor/Qualifier:
Arthralgia / diagnosis*,  etiology,  physiopathology
Arthrography / methods*
Biological Markers / blood
Enzyme-Linked Immunosorbent Assay
Female
Follow-Up Studies
Humans
Male
Middle Aged
Osteoarthritis, Knee / blood*,  complications,  radiography
Pain Measurement
Prognosis
Range of Motion, Articular / physiology*
Severity of Illness Index
Transforming Growth Factor beta2 / blood*
Transforming Growth Factor beta3 / blood*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Transforming Growth Factor beta2; 0/Transforming Growth Factor beta3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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