Document Detail


Sertoli cells as a target for reproductive hazards.
MedLine Citation:
PMID:  11021515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Male fertility can be impaired by various toxicants. Some of them are known to target mainly Sertoli cells, which play an essential role in spermatogenesis. In this study, the in vitro response of immature rat Sertoli cells to various environmental pollutants, including pesticides, oestrogenic compounds and heavy metals, has been investigated. Mitochondrial dehydrogenase activity has been used to measure Sertoli cell viability, while production of lactate and secretion of inhibin B have been used as general and specific cell markers. Sertoli cell viability was not affected after 24-h exposure to lindane, DDT, ethinyloestradiol or bisphenol A in the concentration range analysed (up to 100, 25 or 50 microM, respectively). In contrast, mercury(II) (EC50 = 31 microM) and cisplatin (15% decrease in viability at 100 microM) induced some cytotoxic effect. With the exception of the pesticide DDT, all chemicals investigated induced a significant dose-dependent increase in lactate production after 24-h exposure to Sertoli cells. Owing to the cytotoxic effect of mercury(II), lactate levels dropped again at concentrations above 20 microM. The pesticide lindane (but not DDT) and both oestrogens significantly increased the production of the Sertoli cell specific hormone inhibin B without affecting cell viability. In contrast, the heavy metals mercury(II) and platinum(II) markedly decreased inhibin B levels. This sharp decrease was already significant at metal concentrations that reduced Sertoli cell viability only moderately (10-15%). In conclusion, the secretion of lactate and inhibin B by immature rat Sertoli cells seems to be a useful and sensitive marker with which to explore potential Sertoli cell toxicants.
Authors:
T K Monsees; M Franz; S Gebhardt; U Winterstein; W B Schill; J Hayatpour
Related Documents :
7484435 - Endocrine, paracrine and autocrine regulation of testicular steroidogenesis.
2863395 - The role of germinal epithelium and spermatogenesis in the privileged survival of intra...
10431405 - Some considerations on the human leydig cell (immunohistochemical observations).
3003355 - Study using in-vivo binding of 125i-labelled hcg, light and electron microscopy of the ...
19718715 - Caveolin-1 is an important factor for the metastasis and proliferation of human small c...
2551705 - Forskolin inhibits the gs-stimulated adenylate cyclase in rat ascites hepatoma ah66f ce...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  32     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-02-02     Completed Date:  2001-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  239-46     Citation Subset:  IM    
Affiliation:
Centre of Dermatology and Andrology, Justus Liebig University, Giessen, Germany. Thomas.Monsees@derma.med.uni-giessen.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / drug effects
Environmental Pollutants / toxicity*
Estrogens, Non-Steroidal / toxicity
Inhibins / biosynthesis
Lactic Acid / biosynthesis
Male
Metals, Heavy / toxicity
Oxidoreductases / metabolism
Pesticides / toxicity
Rats
Sertoli Cells / cytology,  drug effects*,  physiology
Chemical
Reg. No./Substance:
0/Environmental Pollutants; 0/Estrogens, Non-Steroidal; 0/Metals, Heavy; 0/Pesticides; 50-21-5/Lactic Acid; 57285-09-3/Inhibins; EC 1.-/Oxidoreductases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Spermatogenesis and mutagenicity of environmental hazards: extrapolation of genetic risk from mouse ...
Next Document:  Evaluation of aneuploidy and DNA damage in human spermatozoa: applications in field studies.