Document Detail

Sertoli cell secretion of a factor that inhibits the incorporation of 3H-thymidine into cells in vitro.
MedLine Citation:
PMID:  8056633     Owner:  NLM     Status:  MEDLINE    
Rat Sertoli cells secrete a low molecular weight factor in vitro that inhibits the incorporation of 3H-thymidine into cells. Although the addition of Sertoli cell-conditioned medium (rSCCM) resulted in nearly a threefold stimulation of cell growth, the incorporation of 3H-thymidine was decreased in a dose-dependent manner and did not reflect the increase in cell number. Peritubular cell- and germ cell-conditioned medium did not contain this inhibitory activity. Nor did the conditioned medium from fibroblasts and a variety of cell lines tested. A low molecular weight filtrate of rSCCM (< 1,000 Da) contained virtually all of the 3H-thymidine inhibiting activity, as well as about 50% of the mitogenic activity in the rSCCM. The inhibitory activity was eliminated upon removal of the rSCCM and was not due to either growth inhibition or a toxic effect on cell proliferation.
D J Lamb; S Lee; S Shubhada
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of andrology     Volume:  15     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    1994 Mar-Apr
Date Detail:
Created Date:  1994-09-12     Completed Date:  1994-09-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  110-6     Citation Subset:  IM    
Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Biological Factors / metabolism,  secretion*
Cell Division
Cell Line
Culture Media
Culture Media, Conditioned
Organ Specificity
Sertoli Cells / cytology,  secretion*
Testis / cytology
Thymidine / antagonists & inhibitors,  metabolism*
Grant Support
Reg. No./Substance:
0/Biological Factors; 0/Culture Media; 0/Culture Media, Conditioned; 10028-17-8/Tritium; 50-89-5/Thymidine

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